Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ORIGIN OF HUMAN MAST-CELLS - DEVELOPMENT FROM TRANSPLANTED HEMATOPOIETIC STEM-CELLS AFTER ALLOGENEIC BONE-MARROW TRANSPLANTATION
Autore:
FODINGER M; FRITSCH G; WINKLER K; EMMINGER W; MITTERBAUER G; GADNER H; VALENT P; MANNHALTER C;
Indirizzi:
UNIV VIENNA,DEPT BIOL MOLEC,CLIN INST MED & CHEM LAB MED,WAEHRINGER GUERTEL 18-20 A-1090 VIENNA AUSTRIA UNIV VIENNA,DEPT BIOL MOLEC,CLIN INST MED & CHEM LAB MED A-1090 VIENNA AUSTRIA UNIV VIENNA,DEPT MED 1,DIV HEMATOL & HEMOSTASEOL VIENNA AUSTRIA ST ANNA CHILDRENS HOSP A-1090 VIENNA AUSTRIA
Titolo Testata:
Blood
fascicolo: 9, volume: 84, anno: 1994,
pagine: 2954 - 2959
SICI:
0006-4971(1994)84:9<2954:OOHM-D>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; VONWILLEBRAND-FACTOR GENE; COLONY-FORMING CELL; RECOMBINANT HUMAN; PROGENITOR CELLS; VARIABLE NUMBER; HUMAN BASOPHILS; KIT-LIGAND; C-KIT; DISEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
M. Fodinger et al., "ORIGIN OF HUMAN MAST-CELLS - DEVELOPMENT FROM TRANSPLANTED HEMATOPOIETIC STEM-CELLS AFTER ALLOGENEIC BONE-MARROW TRANSPLANTATION", Blood, 84(9), 1994, pp. 2954-2959

Abstract

Although mast cells are hematopoietic cells, little is known about the origin of their precursors in vivo. In this study, the origin (donorv recipient genotype) of human mast cells (MCs) was analyzed in a patient who underwent allogeneic bone marrow transplantation (BMT). The patient presented with secondary acute myeloid leukemia (French-American-British classification, M2) arising from refractory anemia with excess of blast cells and bone marrow (BM) mastocytosis. Transplantation was performed in chemotherapy-induced complete remission. On days 88, 126, 198, and 494 after BMT, mast cells were enriched to homogeneity from bone marrow mononuclear cells (BM MNCs) by cell sorting for CD117(+)/CD34(-) cells. Purified mast cell populations were CD117(c-kit)(+) (>95%), CD34(-) (<1%), CD3(-) (<1%), CD14(-) (<1%), and virtually free of contaminating cells as assessed by Giemsa staining. The genotype ofMCs was analyzed after amplification by polymerase chain reaction (PCR) of a variable number tandem repeat (VNTR) region within intron 40 of the von Willebrand factor (VWF) gene. Unexpectedly, on days 88 and 126 after BMT, sorted MCs displayed recipient genotype as shown by vWF.VNTR-PCR. However, on days 198 and 494, PCR analysis showed a switch to donor genotype in isolated mast cells. peripheral brood (PB) and BM MNC as well as highly enriched (sorted) CD3(+) T cells (PB, BM), CB4(+) helper T cells (PB), CD8(+) T cells (PB), CD19(+) B cells (PB), CD14(+) monocytes (PB, BM), and CD34(+) precursor cells (BM) showed donor genotype throughout the observation period. Together, these results provide evidence that human MCs developed in vivo from transplanted hematopoietic stem cells. Engraftment and in vivo differentiation of MCs from early hematopoietic progenitor cells may be a prolonged process. (C) 1994 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:53:42