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Titolo:
USE OF A DEHYDROALANINE-CONTAINING PEPTIDE AS AN EFFICIENT INHIBITOR OF TRIPEPTIDYL PEPTIDASE-II
Autore:
TOMKINSON B; GREHN L; FRANSSON B; ZETTERQVIST O;
Indirizzi:
SWEDISH UNIV AGR SCI,CTR BIOMED,DEPT VET MED CHEM,BOX 575 S-75123 UPPSALA SWEDEN UPPSALA UNIV,DEPT BIOCHEM UPPSALA SWEDEN UPPSALA UNIV,DEPT MED & PHYSIOL CHEM UPPSALA SWEDEN
Titolo Testata:
Archives of biochemistry and biophysics
fascicolo: 2, volume: 314, anno: 1994,
pagine: 276 - 279
SICI:
0003-9861(1994)314:2<276:UOADPA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-ERYTHROCYTES; PURIFICATION; SUBTILISIN; SEQUENCE; ENZYME; SITE; CDNA;
Keywords:
DEHYDROALANINE PEPTIDE; TRIPEPTIDYL PEPTIDASE II; INHIBITOR; BETA-ELIMINATION; SERINE PEPTIDASE; EXOPEPTIDASE; SUBTILISIN TYPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
B. Tomkinson et al., "USE OF A DEHYDROALANINE-CONTAINING PEPTIDE AS AN EFFICIENT INHIBITOR OF TRIPEPTIDYL PEPTIDASE-II", Archives of biochemistry and biophysics, 314(2), 1994, pp. 276-279

Abstract

Tripeptidyl peptidase II is an intracellular exopeptidase, which has been purified from rat liver and human erythrocytes. An efficient specific inhibitor was obtained through beta-elimination of phosphate fromthe phosphopeptide Arg-Ala-Ser(P)-Val-Ala. The dehydroalanine-containing peptide formed was a competitive inhibitor with a K-i of 0.02 +/- 0.01 mu M. This study demonstrated that replacing a serine residue in a good inhibitor with a dehydroalanine residue reduced the K-i 45 times. It is proposed that dehydroalanine-containing peptides could be of interest in the development of inhibitors for other peptidases as well. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 18:39:14