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Titolo:
CHANGE IN GLYCOSYLATION OF CHICKEN TRANSFERRIN GLYCANS BIOSYNTHESIZEDDURING EMBRYOGENESIS AND PRIMARY CULTURE OF EMBRYO HEPATOCYTES
Autore:
JACQUINOT PM; LEGER D; WIERUSZESKI JM; CODDEVILLE B; MONTREUIL J; SPIK G;
Indirizzi:
UNIV SCI & TECH LILLE FLANDRES ARTOIS,CHIM BIOL LAB F-59655 VILLENEUVE DASCQ FRANCE UNIV SCI & TECH LILLE FLANDRES ARTOIS,CNRS,UNITE MIXTE RECH 111 F-59655 VILLENEUVE DASCQ FRANCE
Titolo Testata:
Glycobiology
fascicolo: 5, volume: 4, anno: 1994,
pagine: 617 - 624
SICI:
0959-6658(1994)4:5<617:CIGOCT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAS-LIQUID-CHROMATOGRAPHY; MAGNETIC-RESONANCE SPECTROSCOPY; GLYCOPROTEIN GLYCANS; METHYL GLYCOSIDES; SERUM TRANSFERRIN; HUMAN SEROTRANSFERRIN; STRUCTURAL-ANALYSIS; HEN OVOTRANSFERRIN; MASS-SPECTROMETRY; OLIGOSACCHARIDES;
Keywords:
CHICKEN; EMBRYOGENESIS; EMBRYO HEPATOCYTES; GLYCOSYLATION; TRANSFERRIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
P.M. Jacquinot et al., "CHANGE IN GLYCOSYLATION OF CHICKEN TRANSFERRIN GLYCANS BIOSYNTHESIZEDDURING EMBRYOGENESIS AND PRIMARY CULTURE OF EMBRYO HEPATOCYTES", Glycobiology, 4(5), 1994, pp. 617-624

Abstract

Transferrins were isolated by immunoaffinity chromatography from chicken serum, chicken embryo serum and from the culture medium of chickenembryo hepatocytes in primary culture. The glycovariants of these three transferrins were separated by ion-exchange chromatography using a fast protein liquid chromatography (FPLC) system. The structures of the oligosaccharide-alditols released by hydrazinolysis from the glycovariants were compared after analysis by a combination of methanolysis, methylation analysis and H-1-NMR spectroscopy. In the three transferrins analysed, the oligosaccharides were of the biantennary N-acetyllactosaminic type, having several prominent features. In particular, the embryo serum transferrin glycan differed from that of chicken serum transferrin by the presence of a bisecting N-acetylglucosamine, suggesting a developmental change in glycosylation. The glycan structure of thetransferrin secreted by the embryo hepatocytes in primary culture wasmarked by the presence of fucose (alpha 1-6) linked to the core N-acetylglucosamine, suggesting that expression of the fucosyltransferase activity is dependent on cell culture conditions. Moreover, comparativeanalysis of chicken serum transferrin and ovotransferrin glycans reinforces the idea that the glycosylation of two identical polypeptide chains is organ specific.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:37:22