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Titolo:
PRIMARY CULTURE OF CIRCUMVENTRICULAR ORGANS FROM THE RAT-BRAIN LAMINATERMINALIS
Autore:
JURZAK M; MULLER AR; SCHMID HA; GERSTBERGER R;
Indirizzi:
MAX PLANCK INST PHYSIOL & CLIN RES,WG KERCKHOFF INST,PARKSTR 1 D-61231 BAD NAUHEIM GERMANY
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 662, anno: 1994,
pagine: 198 - 208
SICI:
0006-8993(1994)662:1-2<198:PCOCOF>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTEROVENTRAL 3RD VENTRICLE; ANGIOTENSIN-II; SUBFORNICAL ORGAN; ARGININE-VASOPRESSIN; SUPRAOPTIC NUCLEUS; CELL-CULTURES; NITRIC-OXIDE; NEURONS; DRINKING; LOCALIZATION;
Keywords:
SUBFORNICAL ORGAN; ORGANUM VASCULOSUM OF THE LAMINA TERMINALIS; CELL CULTURE; IMMUNOCYTOCHEMISTRY; NADPH-DIAPHORASE; ANGIOTENSIN II; CALCIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
M. Jurzak et al., "PRIMARY CULTURE OF CIRCUMVENTRICULAR ORGANS FROM THE RAT-BRAIN LAMINATERMINALIS", Brain research, 662(1-2), 1994, pp. 198-208

Abstract

A primary culture system of cells derived from two circumventricular organs (CVO) of the rat brain was established. The subfornical organ (SFO) and the organum vasculosum of the lamina terminalis (OVLT) were dissected from the rostral wall of the third ventricle and its cells taken into culture after mechanical dissociation. The cells were cultured in a modified microculture chamber system ensuring relatively high cell density despite their low absolute number. When animals were injected with Evans blue prior to cell preparation, the macroscopically visible penetration of the dye into the parenchyma of the CVOs could be used as guidance during tissue isolation and labelled cells could be identified in culture. Cultured CVO neurones and astrocytes were identified using antibodies against cell type specific marker proteins. The histochemical NADPH-diaphorase staining was used for the detection of nitric oxide synthase in tissue sections of both CVOs and in their cultured neurones. In addition, angiotensin II (ANG II)-evoked elevations of the intracellular Ca2+ concentration ([Ca2+](i)) in single culturedOVLT neurones were measured. The described methods will be useful forfurther characterization of CVO neurones and astrocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 04:10:09