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Titolo:
SENSITIVITY AND SPECIFICITY OF IN-SITU BACTERIAL CHAIN-REACTION (BCR)IN DETECTING SPARSE HUMAN TUMOR-CELLS IN PERIPHERAL-BLOOD
Autore:
ROTMAN B; GUZMAN R; CRAIG AJ;
Indirizzi:
BROWN UNIV,DEPT MOL BIOL CELL BIOL & BIOCHEM,DIV BIOL & MED PROVIDENCE RI 02912 BCR DIAGNOST INC JAMESTOWN RI 02835
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 1, volume: 229, anno: 1996,
pagine: 80 - 85
SICI:
0006-291X(1996)229:1<80:SASOIB>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW; ANTIBODIES; CARCINOMA; CANCER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
11
Recensione:
Indirizzi per estratti:
Citazione:
B. Rotman et al., "SENSITIVITY AND SPECIFICITY OF IN-SITU BACTERIAL CHAIN-REACTION (BCR)IN DETECTING SPARSE HUMAN TUMOR-CELLS IN PERIPHERAL-BLOOD", Biochemical and biophysical research communications, 229(1), 1996, pp. 80-85

Abstract

We report here the development of bacterial chain reaction (BCR), a system using micro organisms as nanodevices to amplify and visualize signals from molecular bioprobes such as antibodies, binding proteins, lectins, and oligonucleotides. Unlike conventional enzyme-linked amplification systems in which the amount of enzyme is a constant parameter,in the BCR an enzyme (penicillinase) is used to trigger a proliferative chain reaction producing an exponential increase in enzyme. The detection limits and specificity of BCR were determined using a model system designed to detect and enumerate MCF-7 (a human breast adenocarcinoma cell line) cells disseminated at extremely low frequency (e.g., one tumor cell per million normal cells) among monocluclear cells (MNCs)of human peripheral blood. Results of testing 83 specimens of peripheral blood from presumably healthy donors showed 97.6% specificity The system was capable of detecting tumor cells at a frequency of 2x10(-7). (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 16:38:59