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Titolo:
PARTIAL-PURIFICATION AND CHARACTERIZATION OF NUCLEAR RIBONUCLEASE-P FROM WHEAT
Autore:
ARENDS S; SCHON A;
Indirizzi:
UNIV WURZBURG,INST BIOCHEM,BIOZENTRUM,AM HUBLAND D-97074 WURZBURG GERMANY UNIV WURZBURG,INST BIOCHEM,BIOZENTRUM D-97074 WURZBURG GERMANY
Titolo Testata:
European journal of biochemistry
fascicolo: 2, volume: 244, anno: 1997,
pagine: 635 - 645
SICI:
0014-2956(1997)244:2<635:PACONR>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOCHONDRIAL RNASE-P; SCHIZOSACCHAROMYCES-POMBE; SUBSTRATE RECOGNITION; GENOME ORGANIZATION; NICOTIANA-RUSTICA; SEQUENCE ANALYSES; ESCHERICHIA-COLI; GERM EXTRACTS; M1 RNA; IDENTIFICATION;
Keywords:
ENZYME PURIFICATION; NUCLEAR RIBONUCLEASE P; PRE-TRANSFER-RNA PROCESSING; SUBSTRATE SPECIFICITY; TRITICUM AESTIVUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
S. Arends e A. Schon, "PARTIAL-PURIFICATION AND CHARACTERIZATION OF NUCLEAR RIBONUCLEASE-P FROM WHEAT", European journal of biochemistry, 244(2), 1997, pp. 635-645

Abstract

Ribonuclease P (RNase P) from wheat nuclei has been purified over 1000-fold, using wheat germ extract as starting material and a combination of poly(ethylenglycol) precipitation and column chromatography. The enzyme was shown to be of nuclear origin by its characteristic ionic requirements; for optimum activity it requires 0.5-1.5 mM Mg2+, which can be partly replaced by Mn2+. With about 100 kDa, wheat nuclear RNaseP has the lowest molecular mass reported so far for a eukaryotic RNase P. The enzyme has an isoelectric point of 5.0 and a buoyant density of 1.34 g/ml in CsCl, suggesting the presence of a nucleic acid component; it is, however, insensitive against treatment with micrococcal nuclease. Wheat germ RNase P requires an intact tertiary structure of the pre-tRNA substrate; its cleavage efficiency is also influenced by the presence of an intron, and by the nature of the 3' terminus of the substrate. The apparent K-m and V-max for an intronless plant pre-tRNA(Tyr) are 10.3 nM and 1.12 fmol/min, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 21:39:33