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Titolo:
SUBSTRATE-BINDING AND CATALYTIC ROLES OF LYS194 IN THE C-TERMINUS IN HUMAN ADENYLATE KINASE BY SITE-DIRECTED RANDOM MUTAGENESIS
Autore:
AYABE T; TAKENAKA H; TAKENAKA O; ONITSUKA T; SHIBATA K; UESUGI S; HAMADA M;
Indirizzi:
MIYAZAKI MED COLL,DEPT HYG MIYAZAKI 88916 JAPAN MIYAZAKI MED COLL,DEPT SURG 2 MIYAZAKI 88916 JAPAN KYOTO UNIV,PRIMATE RES INST INUYAMA AICHI 484 JAPAN YOKOHAMA NATL UNIV,SCH ENGN,DEPT BIOENGN YOKOHAMA KANAGAWA 240 JAPAN
Titolo Testata:
Biochemistry and molecular biology international
fascicolo: 2, volume: 41, anno: 1997,
pagine: 367 - 375
SICI:
1039-9712(1997)41:2<367:SACROL>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOSINE-TRIPHOSPHATE TRANSPHOSPHORYLASES; GTP-AMP PHOSPHOTRANSFERASE; AMINO-ACID SEQUENCE; CRYSTALLINE RABBIT; CALF MUSCLE; LIVER; CDNA; CLONING;
Keywords:
ADENYLATE KINASE; SITE-DIRECTED MUTAGENESIS; C-TERMINAL DOMAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
T. Ayabe et al., "SUBSTRATE-BINDING AND CATALYTIC ROLES OF LYS194 IN THE C-TERMINUS IN HUMAN ADENYLATE KINASE BY SITE-DIRECTED RANDOM MUTAGENESIS", Biochemistry and molecular biology international, 41(2), 1997, pp. 367-375

Abstract

Site-directed random mutagenesis of Lys194 residue in the C-terminus of human adenylate kinase (AK) was performed, and six mutants were analyzed by steady-state kinetics. K194-mutants variously affected the apparent Michaelis constants (K-m values) for ATP and AMP, although the k(cat) values strikingly decreased. The Lys194 residue appears to interact not only with MgATP(2-) but also with the AMP(2-) substrates by salt bridge formation with a nucleotide and to play a functional role in stabilizing the phosphate-transfer during catalysis. Lys194 could beessential for substrate-holdings and in catalysis and not replaceableto the other amino acids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 18:39:55