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Titolo:
BROMIDE, IN THE THERAPEUTIC CONCENTRATION, ENHANCES GABA-ACTIVATED CURRENTS IN CULTURED NEURONS OF RAT CEREBRAL-CORTEX
Autore:
SUZUKI S; KAWAKAMI K; NAKAMURA F; NISHIMURA S; YAGI K; SEINO M;
Indirizzi:
SHIZUOKA HIGASHI HOSP,NATL EPILEPSY CTR,886 URUSHIYAMA SHIZUOKA 420 JAPAN
Titolo Testata:
Epilepsy research
fascicolo: 2, volume: 19, anno: 1994,
pagine: 89 - 97
SICI:
0920-1211(1994)19:2<89:BITTCE>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIPPOCAMPAL PYRAMIDAL CELLS; SPINAL NEURONS; CHANNELS; EPILEPSY;
Keywords:
BROMIDE; GABA; ANTIEPILEPTIC DRUGS; WHOLE-CELL VOLTAGE-CLAMP RECORDING; CULTURED CORTICAL NEURONS OF RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
S. Suzuki et al., "BROMIDE, IN THE THERAPEUTIC CONCENTRATION, ENHANCES GABA-ACTIVATED CURRENTS IN CULTURED NEURONS OF RAT CEREBRAL-CORTEX", Epilepsy research, 19(2), 1994, pp. 89-97

Abstract

We investigated the effect of bromide on gamma-aminobutyric acid (GABA)-activated currents in cultured cerebral neurons of the rat, employing whole-cell voltage- and current-clamp techniques. Application of 100 mu M GABA elicited currents whose reversal potential was 0 mV with equal concentrations of chloride in both pipette and bath solutions andmore negative than -60 mV with 159 mM chloride extracellularly and 4 mM chloride inside. Bicuculline blocked the currents. These findings showed that the currents were composed of chloride flux through GABA(A)receptor-coupled channels. Reversal potential revealed a permeabilityratio of bromide with respect to chloride (P-Br/P-Cl) of 1.51. When 100 mu M GABA was applied with the extracellular solution containing 140 mM bromide and 19 mM chloride, the currents were enhanced 2.00- and 1.91-fold at the holding potentials of -20 mV and 0 mV, respectively. Extracellular solutions containing various concentrations of bromide substituted for the same amount of chloride were applied with 100 mu M GABA. The therapeutic concentration of 10 mM and 20 mM bromide enhanced the currents 1.28- and 1.36-fold of the control currents at the holding potential of -20 mV, respectively. Under current-damp recording, alarger hyperpolarization was obtained by the application of GABA witha 140 mM bromide-containing solution. These findings suggest that bromide potentiated GABA-activated currents at the therapeutic concentrations ranging from 10 mM to 20 mM, causing the larger GABA-induced hyperpolarization. It is postulated that the antiepileptic effect of bromide might occur through the potentiation of inhibitory postsynaptic potentials elicited by GABA.

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Documento generato il 30/11/20 alle ore 16:41:05