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Titolo:
IN-VITRO EFFECTS OF AMINO-ACID-BASED VERSUS GLUCOSE-BASED CONTINUOUS AMBULATORY PERITONEAL-DIALYSIS FLUIDS ON PERITONEAL MACROPHAGE FUNCTION
Autore:
SCHENK U; KIEFER T; HUBEL E; WEBER J; METTANG T; PASSLICKDEETJEN J; KUHLMANN U;
Indirizzi:
ROBERT BOSCH KRANKENHAUS,DEPT INTERNAL MED,DIV NEPHROL,AUERBACHSTR 110 D-70376 STUTTGART GERMANY FRESENIUS AG OBERURSEL GERMANY
Titolo Testata:
Nephron
fascicolo: 3, volume: 68, anno: 1994,
pagine: 338 - 346
SICI:
0028-2766(1994)68:3<338:IEOAVG>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-ENDOTHELIAL CELLS; HUMAN POLYMORPHONUCLEAR LEUKOCYTES; DEPENDENT CHEMI-LUMINESCENCE; DEFENSE-MECHANISMS; SUPEROXIDE; INVITRO; CAPD; PHAGOCYTOSIS; TOXICITY; REPLICATION;
Keywords:
PERITONEAL DIALYSATE; AMINO ACIDS; OXIDATIVE METABOLISM; BACTERIAL KILLING; PHAGOCYTOSIS; LACTATE; PERITONEAL MACROPHAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
U. Schenk et al., "IN-VITRO EFFECTS OF AMINO-ACID-BASED VERSUS GLUCOSE-BASED CONTINUOUS AMBULATORY PERITONEAL-DIALYSIS FLUIDS ON PERITONEAL MACROPHAGE FUNCTION", Nephron, 68(3), 1994, pp. 338-346

Abstract

We studied the in vitro effects of peritoneal dialysis fluids (PDF) containing 1 and 1.5% amino acids (AA) as compared to approximately equiosmolar glucose (GLU)-based PDF (1.5 and 4.25%) and control buffer, respectively, on peritoneal macrophage (PMO) function. The media were tested at original pH (5.3-5.5) and after pH adjustment to 7.4. PMO were isolated from the effluents of 10 on continuous ambulatory peritoneal dialysis (CAPD) patients and tested for luminol- and lucigenin-enhanced chemiluminescence (CL), superoxide generation measured by cytochrome c reduction, killing capacity and phagocytosis after incubation (30min, 37 degrees C) in the PDF used. All AA-based PDF exhibited a statistically significant depressive effect on integral CL response, O-2(-) production and bacterial killing of PMO at pH 7.4 in comparison withpH-adjusted GLU-based PDF of similar osmolality and buffer. Exposure of PMO to acidic AA-based media did not result in a significantly different suppression of the oxidative metabolism and the killing capacityas compared to fresh GLU-based fluids. Phagocytosis of PMO did not show significant differences after incubation in the solutions studied. Thus, the AA-based PDF employed compromise the oxidative metabolism and the killing capacity of PMO at pH 7.4 in vitro significantly more than GLU-based fluids. Since pH-identical and almost equiosmolar PDF were compared, the specific composition of the AA-based fluids, especially the high content of lactate and several essential AA, could be responsible for this detrimental impact.

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Documento generato il 01/12/20 alle ore 10:00:28