Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CONTRACTILE HYPERREACTIVITY AND ALTERATION OF PKC ACTIVITY IN GASTRICFUNDUS SMOOTH-MUSCLE OF DIABETIC RATS
Autore:
SAKAI Y; INAZU M; SHAMOTO A; ZHU BH; HOMMA I;
Indirizzi:
SHOWA UNIV,SCH MED,DEPT PHYSIOL 2 TOKYO 142 JAPAN
Titolo Testata:
Pharmacology, biochemistry and behavior
fascicolo: 3, volume: 49, anno: 1994,
pagine: 669 - 674
SICI:
0091-3057(1994)49:3<669:CHAAOP>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; PHORBOL ESTER; CALCIUM; STREPTOZOTOCIN; ACTIVATION; REACTIVITY;
Keywords:
DIABETES; PROTEIN KINASE C; GASTRIC SMOOTH MUSCLE; CA2+ SIGNAL; H-7;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
Y. Sakai et al., "CONTRACTILE HYPERREACTIVITY AND ALTERATION OF PKC ACTIVITY IN GASTRICFUNDUS SMOOTH-MUSCLE OF DIABETIC RATS", Pharmacology, biochemistry and behavior, 49(3), 1994, pp. 669-674

Abstract

Contraction dose dependently induced in gastric smooth muscle of diabetic rats by Bay K 8644 in the presence of 20 mM KCl was about two times that induced in controls, and was inhibited more than 50% by 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7). Contraction was caused in diabetics but usually not in controls by 10(-5) M phorbol 12-myristate 13-acetate (PMA). In diabetics, this contraction was about 2.5 times that in controls. Protein kinase C (PKC) activity in the soluble fraction was depressed by H-7 or staurosporine, and depended on PMA concentration, but was greater in diabetics than in controls at any PMA concentration. PKC activity in the soluble fraction was inhibited by lower Ca2+ concentration, and was greater in diabetics than in controls. Affinity and density of binding sites of a Ca2+ channel antagonist ligand, [H-3]PN200-110, were the same in plasma membrane-enriched fractions isolated from either controls or diabetic preparations. Thus, hyperreactivity in diabetic fundus may depend, in part, on alteration of PKC properties, but not on the density of Ca2+ channels.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 02:06:43