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Titolo:
ROUTINE IDENTIFICATION OF MYCOBACTERIUM-TUBERCULOSIS COMPLEX ISOLATESBY AUTOMATED HYBRIDIZATION
Autore:
MABILAT C; DESVARENNE S; PANTEIX G; MACHABERT N; BERNILLON MH; GUARDIOLA G; CROS P;
Indirizzi:
ECOLE NORMALE SUPER LYON,SONDES FROIDES BIOMERIEUX LAB,46 ALLEE ITALIE F-69364 LYON 07 FRANCE BIOMERIEUX SA F-69280 MARCY LETOILE FRANCE INST PASTEUR F-69365 LYON 07 FRANCE
Titolo Testata:
Journal of clinical microbiology
fascicolo: 11, volume: 32, anno: 1994,
pagine: 2702 - 2705
SICI:
0095-1137(1994)32:11<2702:RIOMCI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA; AMPLIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
C. Mabilat et al., "ROUTINE IDENTIFICATION OF MYCOBACTERIUM-TUBERCULOSIS COMPLEX ISOLATESBY AUTOMATED HYBRIDIZATION", Journal of clinical microbiology, 32(11), 1994, pp. 2702-2705

Abstract

Methodologies for biochemical identification of mycobacteria isolatedfrom clinical samples are still cumbersome, taking skilled technicians 3 to 6 weeks. We describe here a 2-h identification system for mycobacterial isolates belonging to the Mycobacterium tuberculosis complex using a DNA probe. After 30 min of hands-off sample preparation, the 1.5-h hybridization test is totally automated in the newly developed VIDAS system (bioMerieux, Marcyl'Etoile, France), which performs solid-phase specific hybridization of 16S rRNA at 37 degrees C. The strain collection of actinomycetes tested was composed of 662 isolates from 27 species: 461 members of the M. tuberculosis complex (443 M. tuberculosis, 10 M. bovis, and 8 M. bovis BCG isolates) and 201 isolates of other species, including 55 M. avium-intracellulare isolates). They were identified by traditional methods: growth rate, colonial morphology, pigmentation, and biochemical profiles. The automated probe assay displayed an excellent correlation with the reference results. The four members of the Nocardia and Rhodococcus genera tested did not cross-hybridize. This flexible random-access and automated technology was shown tosuit the routine context of the laboratory by rapidly delivering the results.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:49:41