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Titolo:
PROPERTIES OF COCKSFOOT STREAK AND COCKSFOOT CRYPTIC, 2 VIRUSES INFECTING COCKSFOOT (DACTYLIS-GLOMERATA) IN SCOTLAND (REPRINTED FROM VOL 124, PG 267-281, 1994)
Autore:
TORRANCE L; JONES AT; DUNCAN GH;
Indirizzi:
SCOTTISH CROP RES INST DUNDEE DD2 5DA SCOTLAND
Titolo Testata:
Annals of Applied Biology
fascicolo: 3, volume: 124, anno: 1994,
pagine: 579 - 593
SICI:
0003-4746(1994)124:3<579:POCSAC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE-STRANDED-RNA; POLYACRYLAMIDE GELS; ELECTRON-MICROSCOPY; PLANT-VIRUSES; ELECTROPHORESIS; PARTICLES;
Keywords:
COCKSFOOT STREAK POTYVIRUS; COCKSFOOT CRYPTIC VIRUS; CRYPTOVIRUSES; DSRNA; DACTYLIS GLOMERATA; ELISA; SEED TRANSMISSION; APHID TRANSMISSION;
Tipo documento:
Reprint
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
L. Torrance et al., "PROPERTIES OF COCKSFOOT STREAK AND COCKSFOOT CRYPTIC, 2 VIRUSES INFECTING COCKSFOOT (DACTYLIS-GLOMERATA) IN SCOTLAND (REPRINTED FROM VOL 124, PG 267-281, 1994)", Annals of Applied Biology, 124(3), 1994, pp. 579-593

Abstract

A Scottish isolate of cocksfoot streak virus (CSV-S) was found to have flexuous filamentous particles which, in sap of infected cocksfoot plants, had a modal length of 712 nm. It was transmitted from infected to healthy cocksfoot plants in a non-persistent manner by Myzus persicae and by mechanical inoculation of infective sap extracts containing an anti-oxidant. Apart from cocksfoot, mechanical inoculation of infective sap succeeded in infecting only four of 22 plant species tested. The infectivity of sap extracts containing 0.2% thioglycerol was lost after heating for 10 min at 55 degrees C but not 50 degrees C, storageat room temperature for 48 but not 24 hours, and after diluting 10(-2) to 10(-3). Highly purified preparations of CSV-S particles sedimented as a single component with a sedimentation coefficient of 139S and had a buoyant density in rubidium bromide of 1.31 g/cm(3). Virus particles were composed of one protein and one ssRNA species with estimated M(r) of 31 000 and 3.2 x 10(6) respectively. In ELISA, an antiserum prepared to CSV-S detected the virus in all aerial parts of infected cocksfoot plants and, when present in the ratio of 1 infected leaf : 1000healthy leaves. Both CSV-S-infected and -uninfected cocksfoot also contained a previously undescribed virus with isometric particles c. 30 nm in diameter. This virus, named cocksfoot cryptic virus (CCV), was seed-borne in two cvs of cocksfoot tested and its particles contained two dsRNA species of estimated M(r) of 1.14 x 10(6) and 1.27 x 10(6). Despite the fact that particles of CSV-S were largely free from CCV particles following exclusion chromatography on agarose beads prior to immunisation, immunoelectron microscopy (IEM) showed that the antiserum prepared to CSV-S also contained some antibodies to CCV. Evidence fromIEM suggested a possible distant serological relationship of CCV to ryegrass and beet (BCV 1 or BCV 2, or both) cryptoviruses, all members of sub-group A of cryptoviruses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:05:51