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Titolo:
THE GATA-4 TRANSCRIPTION FACTOR TRANSACTIVATES THE CARDIAC MUSCLE-SPECIFIC TROPONIN-C PROMOTER-ENHANCER IN NONMUSCLE CELLS
Autore:
IP HS; WILSON DB; HEIKINHEIMO M; TANG ZH; TING CN; SIMON MC; LEIDEN JM; PARMACEK MS;
Indirizzi:
UNIV CHICAGO,DEPT MED,MC 6088,ROOM G-611,5841 S MARYLAND AVE CHICAGO IL 60637 UNIV CHICAGO,DEPT MED CHICAGO IL 60637 UNIV CHICAGO,DEPT MOLEC GENET & CELL BIOL CHICAGO IL 60637 UNIV CHICAGO,DEPT PATHOL CHICAGO IL 60637 UNIV CHICAGO,HOWARD HUGHES MED INST CHICAGO IL 60637 WASHINGTON UNIV,SCH MED,DEPT PEDIAT ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT MOLEC BIOL & PHARMACOL ST LOUIS MO 63110 UNIV HELSINKI,CHILDRENS HOSP HELSINKI FINLAND
Titolo Testata:
Molecular and cellular biology
fascicolo: 11, volume: 14, anno: 1994,
pagine: 7517 - 7526
SICI:
0270-7306(1994)14:11<7517:TGTFTT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYOGENIC REGULATORY FACTORS; LEUCINE ZIPPER PROTEIN; DNA-BINDING PROTEINS; GLOBIN GENE; MYOD FAMILY; RETINOBLASTOMA PROTEIN; TARGETED MUTATION; MYOCARDIAL-CELLS; DEVELOPING HEART; MAMMALIAN-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
83
Recensione:
Indirizzi per estratti:
Citazione:
H.S. Ip et al., "THE GATA-4 TRANSCRIPTION FACTOR TRANSACTIVATES THE CARDIAC MUSCLE-SPECIFIC TROPONIN-C PROMOTER-ENHANCER IN NONMUSCLE CELLS", Molecular and cellular biology, 14(11), 1994, pp. 7517-7526

Abstract

The unique contractile phenotype of cardiac myocytes is determined bythe expression of a set of cardiac muscle-specific genes. By analogy to other mammalian developmental systems, it is likely that the coordinate expression of cardiac genes is controlled by lineage-specific transcription factors that interact with promoter and enhancer elements in the transcriptional regulatory regions of these genes. Although previous reports have identified several cardiac muscle-specific transcriptional elements, relatively little is known about the lineage-specifictranscription factors that regulate these elements. In this report, we demonstrate that the slow/cardiac muscle-specific troponin C (cTnC) enhancer contains a specific binding site for the lineage-restricted zinc finger transcription factor GATA-4 This GATA-4-binding site is required for enhancer activity in primary cardiac myocytes. Moreover, thecTnC enhancer can be transactivated by overexpression of GATA-4 in non-cardiac muscle cells such as NIH 3T3 cells. In situ hybridization studies demonstrate that GATA-4 and cTnC have overlapping patterns of expression in the hearts of postimplantation mouse embryos and that GATA-4 gene expression precedes cTnC expression. Indirect immunofluorescence reveals GATA-4 expression in cultured cardiac myocytes from neonatal rats. Taken together, these results are consistent with a model in which GATA-4 functions to direct tissue-specific gene expression duringmammalian cardiac development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 05:16:59