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Titolo:
HIGH-LEVEL EXPRESSION OF ACTIVE HUMAN CYCLOOXYGENASE-2 IN INSECT CELLS
Autore:
CROMLISH WA; PAYETTE P; CULP SA; OUELLET M; PERCIVAL MD; KENNEDY BP;
Indirizzi:
MERCK FROSST CANADA INC,MERCK FROSST CTR THERAPEUT RES,DEPT BIOCHEM &MOLEC BIOL,POB 1005 POINTE CLAIRE H9R 4P8 PQ CANADA MERCK FROSST CANADA INC,MERCK FROSST CTR THERAPEUT RES,DEPT BIOCHEM &MOLEC BIOL POINTE CLAIRE H9R 4P8 PQ CANADA
Titolo Testata:
Archives of biochemistry and biophysics
fascicolo: 1, volume: 314, anno: 1994,
pagine: 193 - 199
SICI:
0003-9861(1994)314:1<193:HEOAHC>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONSTEROIDAL ANTIINFLAMMATORY DRUGS; PROSTAGLANDIN G/H SYNTHASE; MESSENGER-RNA; GENE; TIS10; PURIFICATION; PROTEINS; CLONING; SYSTEM; CDNA;
Keywords:
CYCLOOXYGENASE-2; OVEREXPRESSION; BACULOVIRUS; NSAIDS; GLYCOSYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
W.A. Cromlish et al., "HIGH-LEVEL EXPRESSION OF ACTIVE HUMAN CYCLOOXYGENASE-2 IN INSECT CELLS", Archives of biochemistry and biophysics, 314(1), 1994, pp. 193-199

Abstract

Active human cyclooxygenase-2 (Cox-2) was expressed at high levels ininsect cells using a recombinant baculovirus. The specific activity of Cox-2 in the microsomes of infected cells was 0.51 mu mol O-2/min/mgand was comparable to that obtained for partially purified Cox-2 fromovine placenta (0.55 mu mol O-2/min/mg). The Cox-2 enzyme expressed in insect cells was glycosylated to varying extents and most of the cyclooxygenase activity was in the high-speed microsomal pellet. The insect-cell-expressed enzyme also showed characteristic 15-hydroxyeicosatetraenoic acid production after aspirin treatment and had typical inhibition profiles with a number of known nonsteroidal antiinflammatory drugs. (C) 1994 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:11:03