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Titolo:
THE EFFECT OF HYPEROSMOLALITY ON THE RATE OF HEAT-PRODUCTION OF QUIESCENT TRABECULAE ISOLATED FROM THE RAT-HEART
Autore:
LOISELLE DS; STIENEN GJM; VANHARDEVELD C; VANDERMEULEN ET; ZAHALAK GI; DAUT J; ELZINGA G;
Indirizzi:
UNIV AUCKLAND,SCH MED,DEPT PHYSIOL AUCKLAND 1030 NEW ZEALAND FREE UNIV AMSTERDAM,FYSIOL LAB NL-1081 BT AMSTERDAM NETHERLANDS WASHINGTON UNIV,DEPT MECH ENGN ST LOUIS MO 63130 UNIV MARBURG,INST NORMALE & PATHOL PHYSIOL D-35033 MARBURG GERMANY
Titolo Testata:
The Journal of general physiology
fascicolo: 6, volume: 108, anno: 1996,
pagine: 497 - 514
SICI:
0022-1295(1996)108:6<497:TEOHOT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SKINNED MUSCLE-FIBERS; SKELETAL-MUSCLE; GUINEA-PIG; HYPERTONIC SOLUTIONS; VENTRICULAR MUSCLE; EXTRACELLULAR MEDIUM; INTRACELLULAR CA2+; FORCE DEVELOPMENT; CARDIAC MYOCYTES; VARIED TONICITY;
Keywords:
HYPERTONICITY; CARDIAC BASAL METABOLISM; CHEMICAL SKINNING; INTRACELLULAR CALCIUM; PROCAINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
79
Recensione:
Indirizzi per estratti:
Citazione:
D.S. Loiselle et al., "THE EFFECT OF HYPEROSMOLALITY ON THE RATE OF HEAT-PRODUCTION OF QUIESCENT TRABECULAE ISOLATED FROM THE RAT-HEART", The Journal of general physiology, 108(6), 1996, pp. 497-514

Abstract

We have measured the rate of heat production of isolated, quiescent, right ventricular trabeculae of the rat under isosmotic and hyperosmotic conditions, using a microcalorimetric technique. In parallel experiments, we measured force production and intracellular calcium concentration ([Ca2+](i)). The rate of resting heat production under isosmoticconditions (mean+/-SEM, n=32) was 100+/-7 mW (g dry wt)(-1); it increased sigmoidally with osmolality, reaching a peak that was about four times the isosmotic value at about twice normal osmotic pressure. The hyperosmotic thermal response was: (a) abolished by anoxia, (b) attenuated by procaine, (c) insensitive to verapamil, ouabain, and external calcium concentration, and (d) absent in chemically skinned trabeculaebathed in low-Ca2+ ''relaxing solution. '' Active force production wasinhibited at all osmolalities above isosmotic. Passive (tonic) force increased to, at most, 15% of the peak active force developed under isosmotic conditions while [Ca2+](i) increased, at most, 30% above its isosmotic value. We infer that hyperosmotic stimulation of resting cardiac heat production reflects, in larPe Dart. greatly increased activity of the sarcoplasmic reticular Ca2+ ATPase bi in the face of increased efflux via a procaine-inhibitable Ca2+-release channel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 18:20:14