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Titolo:
IDENTIFICATION OF A SUBSTANCE, PREVIOUSLY SHOWN TO ENHANCE MITOGENESIS OF HUMAN-LYMPHOCYTES, AS THE ACETAMIDE OF P-AMINOBENZOIC ACID
Autore:
BARBIERI B; PAPADOGIANNAKIS N; ENEROTH P; HANSSON C; ROEPSTORFF P; OLDING LB;
Indirizzi:
HUDDINGE UNIV HOSP,KAROLINSKA INST,DEPT PATHOL S-14186 HUDDINGE SWEDEN HUDDINGE UNIV HOSP,KAROLINSKA INST,APPL BIOCHEM UNIT S-14186 HUDDINGESWEDEN ODENSE UNIV,DEPT MOLEC BIOL DK-5230 ODENSE DENMARK
Titolo Testata:
Biochimica et biophysica acta, L. Lipids and lipid metabolism
fascicolo: 3, volume: 1214, anno: 1994,
pagine: 309 - 316
SICI:
0005-2760(1994)1214:3<309:IOASPS>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARACHIDONIC-ACID;
Keywords:
PERIPHERAL BLOOD; MONONUCLEAR LEUKOCYTE; ARACHIDONIC ACID; P-AMINOBENZOIC ACID; P-ACETAMIDOBENZOIC ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
B. Barbieri et al., "IDENTIFICATION OF A SUBSTANCE, PREVIOUSLY SHOWN TO ENHANCE MITOGENESIS OF HUMAN-LYMPHOCYTES, AS THE ACETAMIDE OF P-AMINOBENZOIC ACID", Biochimica et biophysica acta, L. Lipids and lipid metabolism, 1214(3), 1994, pp. 309-316

Abstract

We characterize here an arachidonic acid (AA)-derived metabolite previously found to have an adjuvant effect in phytohemagglutinin-induced mitogenesis of lymphocytes from mothers of newborn babies and from immunodeficient infants. We named the metabolite 'compound 4' due to its position in a thin-layer chromatography system developed for isolationof eicosanoids. The compound was originally found to be produced by peripheral blood mononuclear leukocytes and the T cell leukemia line Jurcat after long-term (28-24 h) incubation with [1-C-14]AA. Compound 4 is also produced by lymphocytes, monocytes, platelets, trombocytes, cultured fibroblasts and various types of malignant cell lines. We purified this metabolite by means of high pressure liquid chromatography with synchronous detection of radioactivity and measurement of ultraviolet-light absorption at 278 nm. Proton nuclear magnetic resonance spectroscopy and mass spectrometry with electron impact techniques demonstrated that compound 4 is not an eicosanoid, but is identical to p-acetamidobenzoic acid (PACBA). The cells synthezise PACBA from p-aminobenzoic acid and a two-carbon residue from AA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 09:42:58