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Titolo:
COMPARATIVE-STUDY OF SINGLE AND NESTED PCR FOR THE DETECTION OF PROVIRAL HIV2 DNA
Autore:
LOUSSERTAJAKA I; SIMON F; FARFARA I; HOUHOU N; COUTOFERNANDEZ J; DAZZA MC; BRUNVEZINET F;
Indirizzi:
HOP BICHAT CLAUDE BERNARD,VIROL LAB F-75877 PARIS 18 FRANCE HOP CLAUDE BERNARD,INSERM,U13,IMEA F-75019 PARIS FRANCE
Titolo Testata:
Research in virology
fascicolo: 5, volume: 145, anno: 1994,
pagine: 337 - 342
SICI:
0923-2516(1994)145:5<337:COSANP>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; IMMUNODEFICIENCY-VIRUS TYPE-1; TRANSMISSION; INFECTION;
Keywords:
HIV-2, HIV, PCR, DNA, CD4+; SINGLE AND NESTED PCR, PROVIRAL DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
I. Loussertajaka et al., "COMPARATIVE-STUDY OF SINGLE AND NESTED PCR FOR THE DETECTION OF PROVIRAL HIV2 DNA", Research in virology, 145(5), 1994, pp. 337-342

Abstract

We have tested seven pairs of primers far the detection of HIV2 DNA by single PCR in positive cultures from 21 infected patients. Four of these primer pairs were then used in a comparative study of single and nested PCR for the detection of HIV2 in fresh lymphocytes from 33 patients infected by the virus. HIV2 DNA was detected in 17 of the 33 patients (51.5%) by single PCR and 29/33 (88%) by nested PCR. All the patients negative in both nested and single PCR were asymptomatic and had CD4(+) lymphocyte counts of at least 500x10(6)/l. This lack of PCR sensitivity for the detection of proviral HIV2 DNA in fresh lymphocytes cannot be totally attributed to genetic variability and may be related to a low viral load in asymptomatic HIV2-infected patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:15:53