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Titolo:
THYMIC SELECTION AND PEPTIDE-INDUCED ACTIVATION OF T-CELL RECEPTOR-TRANSGENIC CD8 T-CELLS IN INTERLEUKIN-2-DEFICIENT MICE
Autore:
KRAMER S; MAMALAKI C; HORAK I; SCHIMPL A; KIOUSSIS D; HUNIG T;
Indirizzi:
UNIV WURZBURG,INST VIROL & IMMUNOBIOL,VERSBACHER STR 7 D-97078 WURZBURG GERMANY UNIV WURZBURG,INST VIROL & IMMUNOBIOL D-97078 WURZBURG GERMANY NATL INST MED RES LONDON NW7 1AA ENGLAND
Titolo Testata:
European Journal of Immunology
fascicolo: 10, volume: 24, anno: 1994,
pagine: 2317 - 2322
SICI:
0014-2980(1994)24:10<2317:TSAPAO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODIES; CYTO-TOXICITY; IFN-GAMMA; EXPRESSION; IL-2; DIFFERENTIATION; SUFFICIENT; DEFICIENT; DELETION; MOLECULE;
Keywords:
INTERLEUKIN-2-DEFICIENT; T CELL RECEPTOR-TRANSGENIC MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
S. Kramer et al., "THYMIC SELECTION AND PEPTIDE-INDUCED ACTIVATION OF T-CELL RECEPTOR-TRANSGENIC CD8 T-CELLS IN INTERLEUKIN-2-DEFICIENT MICE", European Journal of Immunology, 24(10), 1994, pp. 2317-2322

Abstract

The requirement for interleukin-2 (IL-2) in repertoire selection and peripheral activation of CD8 T cells was tested in mice rendered IL-2 deficient by gene targeting and expressing a transgenic T cell receptor (TcR) (F5) specific for influenza nucleoprotein (NP) 366-374 + H-2D(b). Positive selection of the transgenic F5 TcR into the CD8 compartment proceeded normally. Both in vivo and in vitro, the antigenic peptide induced depletion of immature thymocytes and proliferation of matureCD8 T cells regardless of the presence of an intact IL-2 gene. In contrast, cytotoxic T lymphocyte (CTL) activity was only generated by T cells from IL-2(+) F5 transgenic mice. Exogenous IL-2 was able to fullyrestore the CTL response of IL-2(-/-) responder cells in vitro. Thus,both in vivo and in vitro, clonal expansion of CD8 T cells can proceed in the absence of IL-2, whereas in peptide-immunized F5 transgenic mice, induction of cytotoxic effector function is IL-2 dependent.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 12:53:03