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Titolo:
THE FUNCTION OF THE HYPUSINE-CONTAINING PROTEINS OF YEAST AND OTHER EUKARYOTES IS WELL CONSERVED
Autore:
MAGDOLEN V; KLIER H; WOHL T; KLINK F; HIRT H; HAUBER J; LOTTSPEICH F;
Indirizzi:
MAX PLANCK INST BIOCHEM,KLOPFERSPITZ 18A D-82152 MARTINSRIED GERMANY MAX PLANCK INST BIOCHEM D-82152 MARTINSRIED GERMANY CHRISTIAN ALBRECHTS UNIV KIEL,INST BIOCHEM D-24118 KIEL GERMANY UNIV VIENNA,INST MIKROBIOL & GENET A-1090 VIENNA AUSTRIA SANDOZ GMBH,RES INST A-1235 VIENNA AUSTRIA TECH UNIV MUNICH,FRAUENKLIN D-81675 MUNICH GERMANY
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 6, volume: 244, anno: 1994,
pagine: 646 - 652
SICI:
0026-8925(1994)244:6<646:TFOTHP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
INITIATION-FACTOR EIF-4D; SACCHAROMYCES-CEREVISIAE; GENE DISRUPTION; CDNA; EXPRESSION; SEQUENCE; OXYGEN; HYP2P; ACID;
Keywords:
SACCHAROMYCES CEREVISIAE; COMPLEMENTATION ANALYSIS; EUKARYOTIC TRANSLATION INITIATION FACTOR EIF-5A; ANAEROBIOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
V. Magdolen et al., "THE FUNCTION OF THE HYPUSINE-CONTAINING PROTEINS OF YEAST AND OTHER EUKARYOTES IS WELL CONSERVED", MGG. Molecular & general genetics, 244(6), 1994, pp. 646-652

Abstract

The hypusine-containing protein (Hypp) is highly conserved in evolution, from man to archaebacteria, but is not found in eubacteria. Hypp is essential for the viability for yeast cells, where two forms are encoded by the genes HYP1 and HYP2. The hypusine-containing protein Hyp2p, encoded by the HYP2 gene in yeast, is present under both aerobic andanaerobic conditions, whereas Hyp1p synthesis is restricted to anaerobiosis. hyp1 disruption mutants grown under anaerobic conditions reveal no detectable alteration in phenotype relative to wild-type strains. We demonstrate that either Hyp1p or Hyp2p alone is sufficient for normal growth under both metabolic conditions. Moreover, Hypp from various eukaryotic species (slime mold, alfalfa and man) carries the lysine to hypusine modification when expressed in yeast and can substitute functionally for Hyp2p in strains disrupted for HYP2, indicating a highly conserved function of this protein. In contrast, the archaebacterialHypp expressed in yeast is neither modified by hypusine, nor does it allow growth of cells deficient for yeast Hypp.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 23:04:07