Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THERMOLYSIN ACTIVATION MUTANTS WITH CHANGES IN THE FUSOGENIC REGION OF AN INFLUENZA-VIRUS HEMAGGLUTININ
Autore:
ORLICH M; ROTT R;
Indirizzi:
UNIV GIESSEN,INST VIROL,FRANKFURTER STR 107 D-35392 GIESSEN GERMANY UNIV GIESSEN,INST VIROL D-35392 GIESSEN GERMANY
Titolo Testata:
Journal of virology
fascicolo: 11, volume: 68, anno: 1994,
pagine: 7537 - 7539
SICI:
0022-538X(1994)68:11<7537:TAMWCI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLEAVAGE SITE; GLYCOPROTEIN; PROTEASE; FUSION; DETERMINANT; PNEUMONIA;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
M. Orlich e R. Rott, "THERMOLYSIN ACTIVATION MUTANTS WITH CHANGES IN THE FUSOGENIC REGION OF AN INFLUENZA-VIRUS HEMAGGLUTININ", Journal of virology, 68(11), 1994, pp. 7537-7539

Abstract

Influenza virus A/seal/Mass/1/80 (H7N7) mutants were obtained; the hemagglutinins (HAs) of the mutants were not activated by trypsin, as inthe wild-type virus, but by thermolysin. The mutants grew efficientlyunder multiple replication cycle conditions and formed plaques in chicken embryo cells only when thermolysin was added to the culture medium. They exhibited hemolytic activity and induced protective immunity in chickens after an asymptomatic course of infection. Nucleotide sequencing of the HA gene and direct amino acid sequencing showed that insertion of a single leucine into the fusion peptide of the HA2 chain close to the cleavage site and a shift of the cleavage site toward the C terminus by one amino acid were responsible for the changes in the biological properties of the thermolysin activation mutants. Revertants could be obtained when trypsin or trypsin-like endoproteases were present in the virus-producing system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 01:32:39