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Titolo:
SCANNING MUTAGENESIS OF THE ARGININE-RICH REGION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REV TRANS ACTIVATOR
Autore:
HAMMERSCHMID M; PALMERI D; RUHL M; JAKSCHE H; WEICHSELBRAUN I; BOHNLEIN E; MALIM MH; HAUBER J;
Indirizzi:
SANDOZ GMBH,RES INST,BRUNNER STR 59 A-1235 VIENNA AUSTRIA SANDOZ GMBH,RES INST A-1235 VIENNA AUSTRIA UNIV PENN,SCH MED,HOWARD HUGHES MED INST PHILADELPHIA PA 19104 UNIV PENN,SCH MED,DEPT MICROBIOL PHILADELPHIA PA 19104 UNIV PENN,SCH MED,DEPT MED PHILADELPHIA PA 19104
Titolo Testata:
Journal of virology
fascicolo: 11, volume: 68, anno: 1994,
pagine: 7329 - 7335
SICI:
0022-538X(1994)68:11<7329:SMOTAR>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIRAL MESSENGER-RNA; HIV-1 REV; GENE-PRODUCT; RESPONSIVE ELEMENT; CARBOXY-TERMINUS; AMINO-ACIDS; I REX; PROTEIN; BINDING; RECOGNITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
M. Hammerschmid et al., "SCANNING MUTAGENESIS OF THE ARGININE-RICH REGION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REV TRANS ACTIVATOR", Journal of virology, 68(11), 1994, pp. 7329-7335

Abstract

The structural proteins of human immunodeficiency virus type 1, for example, Gag and Env, are encoded by unspliced and incompletely splicedviral transcripts. The expression of these mRNAs in the cytoplasm, along with their commensurate translation, is absolutely dependent on the virally encoded Rev trans activator. Previous studies have demonstrated that Rev binds directly to its substrate mRNAs via an arginine-rich element that also serves as its nuclear localization sequence. In anattempt to define the specific amino acid residues that are importantfor in vivo activity, we have constructed a series of missense mutations that scan across this region. Our data demonstrate that all eight arginine residues within this element can, individually, be substituted for either leucine or lysine with no apparent loss of function. Importantly, these findings suggest that no single amino acid within the arginine-rich domain of Rev is, by itself, essential for activity and that considerable functional redundancy is therefore likely to exist within this region. Interestingly, one mutant in which a tryptophan had been substituted for a serine failed to accumulate exclusively in the nucleus but still bound RNA in a manner that was indistinguishable from that of the wild-type protein. This observation indicates that features of the arginine-rich region that are additional to those required for RNA binding are important for Rev's correct accumulation in the nucleus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:40:23