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Titolo:
INFLUENCE OF TUMOR SIZE ON THE MAIN DRUG-METABOLIZING ENZYME-SYSTEMS IN MOUSE COLON ADENOCARCINOMA CO38
Autore:
MASSAAD L; CHABOT GG; TOUSSAINT C; KOSCIELNY S; MORIZET J; BISSERY MC; GOUYETTE A;
Indirizzi:
INST GUSTAVE ROUSSY,DEPT PHARMACOLTOXICOL & PHARMACOGENET,CNRS,URA 147,PAVILLON RECH F-94805 VILLEJUIF FRANCE INST GUSTAVE ROUSSY,DEPT BIOSTAT & EPIDEMIOL F-94805 VILLEJUIF FRANCE RHONE POULENC RORER SA,CTR RECH VITRY ALFORTVILLE VITRY FRANCE
Titolo Testata:
Cancer chemotherapy and pharmacology
fascicolo: 6, volume: 34, anno: 1994,
pagine: 497 - 502
SICI:
0344-5704(1994)34:6<497:IOTSOT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTATHIONE S-TRANSFERASES; HUMAN-LIVER; CYTOCHROME-P-450; PURIFICATION; ACTIVATION; MICROSOMES; MARKER; ACID;
Keywords:
DRUG-METABOLIZING ENZYMES; CO38 TUMORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
L. Massaad et al., "INFLUENCE OF TUMOR SIZE ON THE MAIN DRUG-METABOLIZING ENZYME-SYSTEMS IN MOUSE COLON ADENOCARCINOMA CO38", Cancer chemotherapy and pharmacology, 34(6), 1994, pp. 497-502

Abstract

Mouse colon adenocarcinoma Co38 is widely used as a screening model for human colon tumors. To understand better the influence of tumor size on the main drug-metabolizing enzyme systems, we tested 15 mouse Co38 tumors at different sizes. The average weight was 917 +/- 444 mg (range, 300-1,400 mg). Cytochromes P-450 (1A1/1A2, 2B1/B2, 2C8-10, 2E1, 3A4), epoxide hydrolase (EH), and glutathione-S-transferases (GST-alpha, -mu, and -pi) were assayed by immunoblotting. The activities of the following enzymes or cofactors were determined by spectrophotometric or fluorometric assays: 1-chloro-2,4-dinitrobenzene-GST (CDNB-GST), selenium-independent glutathione peroxidase (GPX), 3,4-dichloronitrobenzene-GST (DCNB-GST), ethacrynic acid-GST (EA-GST), total glutathione (GSH), uridine diphosphate-glucuronosyltransferase (UDP-GT), beta-glucuronidase (beta G), sulfotransferase (ST), and sulfatase (S). Our resultsshowed the absence of all probed P-450s and EH in Co38 tumors. No relationship was found between the Co38 tumor weights and GPX, GST-alpha,and EA-GST (regression analysis). However, a significant correlation was found between the tumor weights and all other enzymes investigated. For certain enzymes or cofactors, a linear decrease (P <0.05) was observed as a function of tumor weight (CDNB-GST, DCNB-GST, GST-mu, GST-pi, GSH, and beta G). Other enzymatic activities (UDP-GT, S, and ST) were found to decrease in medium-size tumors and to increase in large tumors (P <0.05; quadratic correlation). These data demonstrate that the expression of many drug-metabolizing enzyme systems is altered during tumor growth and suggest that tumoral response to chemotherapy couldbe altered as a function of tumor size.

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Documento generato il 24/11/20 alle ore 08:08:22