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Titolo:
PILC OF NEISSERIA-MENINGITIDIS IS INVOLVED IN CLASS-II PILUS FORMATION AND RESTORES PILUS ASSEMBLY, NATURAL TRANSFORMATION COMPETENCE AND ADHERENCE TO EPITHELIAL-CELLS IN PILC-DEFICIENT GONOCOCCI
Autore:
RYLL RR; RUDEL T; SCHEUERPFLUG I; BARTEN R; MEYER TF;
Indirizzi:
MAX PLANCK INST BIOL,INFEKT BIOL ABT,SPEMANNSTR 34 D-72076 TUBINGEN GERMANY MAX PLANCK INST BIOL,INFEKT BIOL ABT D-72076 TUBINGEN GERMANY MAX PLANCK INST INFEKT BIOL,MOL BIOL ABT D-10117 BERLIN GERMANY
Titolo Testata:
Molecular microbiology
fascicolo: 5, volume: 23, anno: 1997,
pagine: 879 - 892
SICI:
0950-382X(1997)23:5<879:PONIII>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
FORMING PROTEIN PILIN; ANTIGENIC VARIATION; ENDOTHELIAL-CELLS; GENE CONVERSION; MONOCLONAL-ANTIBODIES; STRUCTURAL GENE; GONORRHOEAE; VARIANTS; SEQUENCE; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
R.R. Ryll et al., "PILC OF NEISSERIA-MENINGITIDIS IS INVOLVED IN CLASS-II PILUS FORMATION AND RESTORES PILUS ASSEMBLY, NATURAL TRANSFORMATION COMPETENCE AND ADHERENCE TO EPITHELIAL-CELLS IN PILC-DEFICIENT GONOCOCCI", Molecular microbiology, 23(5), 1997, pp. 879-892

Abstract

Type 4 piIi produced by the pathogenic Neisseria species constitute primary determinants for the adherence to host tissues, In addition to the major pilin subunit (PIIE), neisserial piIi contain the variable PiIC proteins represented by two variant gene copies in most pathogenicNeisseria isolates, Based upon structural differences in the conserved regions of PIIE, two pilus classes can be distinguished in Neisseriameningitidis, For class I pill found in both Neisseria gonorrhoeae and N, meningitidis, PiIC proteins have been implicated in pilus assembly, natural transformation competence and adherence to epithelial cells, In this study, we used primers specific for the piIC2 gene of N, gonorrhoeae strain MS11 to amplify, by the polymerase chain reaction, andclone a homologous piIC gene from N. meningitidis strain A1493 which produces class II pill, This gene was sequenced and the deduced amino acid sequence showed 75.4% and 73.8% identity with the gonococcal PiIC1 and PiIC2, respectively, These values match the identity value of 74.1% calculated for the two N. gonorrhoeae MS11 PiIC proteins, indicating a horizontal relationship between the N, gonorrhoeae and N, meningitidis piIC genes, We provide evidence that PiIC functions in meningococcal class II pilus assembly and adherence, Furthermore, expression ofthe cloned N. meningitidis piIC gene in a gonococcal piIC1,2 mutant restores pilus assembly, adherence to ME-180 epithelial cells, and transformation competence to the wild-type level, Thus, PiIC proteins exhibit indistinguishable functions in the context of class I and class IIpiIi.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:46:46