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Titolo:
TRANSFORMING GROWTH-FACTOR-BETA-2 IS THE PREDOMINANT ISOFORM IN THE NEURAL RETINA, RETINAL-PIGMENT EPITHELIUM-CHOROID AND VITREOUS OF THE MONKEY EYE
Autore:
PFEFFER BA; FLANDERS KC; GUERIN CJ; DANIELPOUR D; ANDERSON DH;
Indirizzi:
AMER CYANAMID CO,DIV STORZ,BLDG 96,ROOM 225A,401 N MIDDLETOWN RD PEARL RIVER NY 10965 NEI,RETINAL CELL & MOLEC BIOL LAB BETHESDA MD 20892 NCI,CHEMOPREVENT LAB BETHESDA MD 20892 UNIV CALIF SANTA BARBARA,INST NEUROSCI SANTA BARBARA CA 93106
Titolo Testata:
Experimental Eye Research
fascicolo: 3, volume: 59, anno: 1994,
pagine: 323 - 333
SICI:
0014-4835(1994)59:3<323:TGITPI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED IMMUNOSORBENT ASSAYS; MOLECULAR-WEIGHT COMPLEX; TGF-BETA; IMMUNOHISTOCHEMICAL LOCALIZATION; INTERPHOTORECEPTOR MATRIX; CELL-PROLIFERATION; ENDOTHELIAL-CELLS; BINDING PROTEIN; HUMAN-PLATELETS; IDENTIFICATION;
Keywords:
TRANSFORMING GROWTH FACTOR BETA; RETINAL PIGMENT EPITHELIUM, PHOTORECEPTOR; MONKEY; IMMUNOCYTOCHEMISTRY; EPITHELIAL CELL CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
B.A. Pfeffer et al., "TRANSFORMING GROWTH-FACTOR-BETA-2 IS THE PREDOMINANT ISOFORM IN THE NEURAL RETINA, RETINAL-PIGMENT EPITHELIUM-CHOROID AND VITREOUS OF THE MONKEY EYE", Experimental Eye Research, 59(3), 1994, pp. 323-333

Abstract

Several techniques were utilized to assess the levels, disposition and cellular sources of isoforms 1 and 2 of transforming growth factor beta (TGF-beta) in the posterior pole of the monkey eye. Freshly dissected tissues, as well as the saline vehicles in which dissections were performed, were analysed by sandwich enzyme-linked immunosorbent assay. In all tissues TGF-beta 2 was the predominant isoform, with beta 2:beta 1 ratios of 6:1 for neural retina (as ng g(-1)) and 425:1 for vitreous (as pmol l(-1)). Retinal pigment epithelium (RPE)-Bruch's membrane-choroid complex contained approximately 10 times the amount of both TGF-beta isoforms as neural retina. For first passage cultures of monkey RPE, TGF-beta 2, but not TGF-beta 1, accumulated over time in conditioned media samples. Immunoreactivity for TGF-beta 2 was detected both in tissue sections of posterior pole, specifically in rod outer segments and RPE, and also in the first passage cultures of RPE. Antibodies to specific peptide sequences of both isoforms localized TGF-beta tothe outer segments of rod photoreceptors. The apparent sequestration of TGF-beta 2 in photoreceptor outer segments, as well as the in vitroevidence for possible synthesis and release by RPE, suggest that TGF-beta 2 is an important modulator of visual function acting at the retina-RPE interface.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 22:38:37