Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EXCITOTOXICITY OF GLUTAMATE AND 4 ANALOGS IN PRIMARY SPINAL-CORD CELL-CULTURES
Autore:
WELLS K; ANDERSON DK; FAROOQUI AA; HORROCKS LA;
Indirizzi:
OHIO STATE UNIV,DEPT MED BIOCHEM,1645 NEIL AVE COLUMBUS OH 43210 OHIO STATE UNIV,DEPT MED BIOCHEM COLUMBUS OH 43210 VET ADM MED CTR CINCINNATI OH 45220
Titolo Testata:
Neurochemistry international
fascicolo: 4, volume: 25, anno: 1994,
pagine: 377 - 384
SICI:
0197-0186(1994)25:4<377:EOGA4A>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID RECEPTORS; CORTICAL CULTURE; NEURONAL INJURY; NEUROTOXICITY; NMDA; VIABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
K. Wells et al., "EXCITOTOXICITY OF GLUTAMATE AND 4 ANALOGS IN PRIMARY SPINAL-CORD CELL-CULTURES", Neurochemistry international, 25(4), 1994, pp. 377-384

Abstract

Continuous glutamate exposure produced widespread neuronal damage in mixed whole dissociated murine spinal cord cell cultures. Ethidium bromide and acridine orange staining revealed that a 24 h glutamate exposure produced nearly 98% neuronal cell death but the underlying glia were spared. Continuous exposure to glutamate, N-methyl-D-aspartate (NMDA), kainate and quisqualate produced time-dependent and dose-dependentcell death as measured by the assay of lactate dehydrogenase activityin the cell culture media. Glutamate (500 mu M), NMDA (100 mu M) and kainate (500 mu M) were equally neurotoxic. In contrast, quisqualate (100 mu M) was only partially neurotoxic compared to the other glutamate analogs. The neurotoxicity of glutamate was blocked by the NMDA antagonist, MK-801. The neurotoxicity of kainate and quisqualate was blocked with the non-NMDA antagonist CNQX. Continuous exposure to (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (1S,3R-ACPD) was not neurotoxic, even at concentrations up to 1 mM.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 06:36:28