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Titolo:
DETERMINATION OF BACTERIAL-CELL NUMBER AND CELL-VOLUME BY MEANS OF FLOW-CYTOMETRY, TRANSMISSION ELECTRON-MICROSCOPY, AND EPIFLUORESCENCE MICROSCOPY
Autore:
HELDAL M; NORLAND S; BRATBAK G; RIEMANN B;
Indirizzi:
UNIV BERGEN,DEPT MICROBIOL & PLANT PHYSIOL,JAHNEBAKKEN 5 N-5007 BERGEN NORWAY INT AGCY DETERMINAT 14C,INST WATER QUAL DK-2970 HORSHOLM DENMARK
Titolo Testata:
Journal of microbiological methods
fascicolo: 4, volume: 20, anno: 1994,
pagine: 255 - 263
SICI:
0167-7012(1994)20:4<255:DOBNAC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
MARINE-BACTERIA; ACID SYNTHESIS; IMAGE-ANALYSIS; GROWTH RATES; BACTERIOPLANKTON; BIOMASS; SEAWATER; FILTERS;
Keywords:
BACTERIA; EPIFLUORESCENCE MICROSCOPY; FLOW CYTOMETRY; TRANSMISSION ELECTRON MICROSCOPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
M. Heldal et al., "DETERMINATION OF BACTERIAL-CELL NUMBER AND CELL-VOLUME BY MEANS OF FLOW-CYTOMETRY, TRANSMISSION ELECTRON-MICROSCOPY, AND EPIFLUORESCENCE MICROSCOPY", Journal of microbiological methods, 20(4), 1994, pp. 255-263

Abstract

Comparative measurements of bacterial total counts and volumes by flow cytometry (FCM), transmission electron microscopy (TEM), and epifluorescence microscopy (EFM), were undertaken during a four week mesocosmexperiment. Total counts of bacteria measured by TEM, EFM, and FCM were in the range of 1.10(6)-6.10(6) cells ml(-1), 1.10(6)-3.10(6) cellsml(-l), and 5.10(5) cells ml(-1) respectively. The mean volume of thebacterial community, measured by means of EFM and TEM, increased from0.12-0.15 mu m(3) at the start of the experiment to 0.39-0.53 mu m(3)at the end. Generally, there was good agreement between the two methods and regression analyses gave r = 0.87 (p < < 0.01) for cell volume and r = 0.97 (p < < 0.01) for cell number. DAPI stained bacteria with volumes less than 0.2 mu m(3) were not detected by flow cytometry and these counts were generally an order of magnitude lower than counts made by TEM and EFM. For samples where the mean bacterial cell volume was larger than 0.3 mu m(3), all three methods were in agreement both with respect to counts and volume estimates.

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Documento generato il 15/01/21 alle ore 21:34:14