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Titolo:
EVIDENCE FOR RADICAL SPECIES AS INTERMEDIATES IN CADMIUM ZINC-METALLOTHIONEIN-DEPENDENT DNA-DAMAGE IN-VITRO
Autore:
MULLER T; SCHUCKELT R; JAENICKE L;
Indirizzi:
INST BIOL FORSCH,FUGGERSTR 3 D-51149 COLOGNE 90 GERMANY UNIV COLOGNE,INST BIOCHEM W-5000 COLOGNE GERMANY
Titolo Testata:
Environmental health perspectives
, volume: 102, anno: 1994, supplemento:, 3
pagine: 27 - 29
SICI:
0091-6765(1994)102:<27:EFRSAI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
INVITRO; ZINC; ANTIOXIDANT; KIDNEY; CELLS; LIVER;
Keywords:
CADMIUM ZINC METALLOTHIONEIN; DNA STRAND BREAKS; RADICALS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
T. Muller et al., "EVIDENCE FOR RADICAL SPECIES AS INTERMEDIATES IN CADMIUM ZINC-METALLOTHIONEIN-DEPENDENT DNA-DAMAGE IN-VITRO", Environmental health perspectives, 102, 1994, pp. 27-29

Abstract

Toxicologic data on cadmium (Cd) indicate that intracellular metallothionein (MT) is protective for Cd exposure, whereas extracellular Cd-containing MT might be toxic. Moreover, Cd is suspected to be a carcinogen though the underlying mechanism is not known. Here we report on the genotoxic activity of cadmium/zinc-metallothionein (Cd/Zn-MT) in a cell-free test system: a concentration-dependent: increase in DNA strand breaks was detected with increasing doses of Cd/Zn-MT, whereas no DNA strand breaks were observed in the presence of heat/denatured MT or Cd or Zn ions alone. Modifications of native Cd/Zn-MT by the metal ion-chelating agent EDTA or the sulfhydryl group alkylating agents N-ethylmaleimide and iodoacetamide suggest that the various cysteine residues of MT, together with the attached heavy metal ions, may be involved in the DNA cleavage reaction. Furthermore, DNA strand breaks caused byCd/Zn-MT seem more likely to be random than sequence- or base-specific. Results from experiments with radical scavengers and electron spin resonance spectroscopy point to radical species formed by Cd/Zn-MT as mediators of the DNA damage. Thus, the actual activity of Cd/Zn-MT-whether protective or damaging-appears to depend on various parameters governed by the extra- and intracellular environment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:29:46