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Titolo:
DYNAMIC INTERACTIONS OF THE ASIALOGLYCOPROTEIN RECEPTOR SUBUNITS WITHCOATED PITS - ENHANCED INTERACTIONS OF H2 FOLLOWING ASSOCIATION WITH H1
Autore:
KATZIR Z; NARDI N; GEFFEN I; FUHRER C; HENIS YI;
Indirizzi:
TEL AVIV UNIV,GEORGE S WISE FAC LIFE SCI,DEPT BIOCHEM IL-69978 TEL AVIV ISRAEL TEL AVIV UNIV,GEORGE S WISE FAC LIFE SCI,DEPT BIOCHEM IL-69978 TEL AVIV ISRAEL UNIV BASEL,BIOCTR,DEPT BIOCHEM CH-4056 BASEL SWITZERLAND
Titolo Testata:
The Journal of biological chemistry
fascicolo: 34, volume: 269, anno: 1994,
pagine: 21568 - 21575
SICI:
0021-9258(1994)269:34<21568:DIOTAR>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE PHOTOBLEACHING RECOVERY; LYSOSOMAL ACID-PHOSPHATASE; CYTOPLASMIC DOMAIN; TRANSFERRIN RECEPTOR; PLASMA-MEMBRANE; LATERAL MOBILITY; INTERNALIZATION SIGNALS; MEDIATED ENDOCYTOSIS; INVITRO BINDING; AP-2 SUBUNIT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
74
Recensione:
Indirizzi per estratti:
Citazione:
Z. Katzir et al., "DYNAMIC INTERACTIONS OF THE ASIALOGLYCOPROTEIN RECEPTOR SUBUNITS WITHCOATED PITS - ENHANCED INTERACTIONS OF H2 FOLLOWING ASSOCIATION WITH H1", The Journal of biological chemistry, 269(34), 1994, pp. 21568-21575

Abstract

Lateral mobility studies comparing native and mutated membrane proteins, combined with treatments that alter clatbrin lattice structure, can measure membrane protein-coated pit interactions in intact cells (Fire, E., Zwart, D., Roth, M.G., an Henis, Y.I. (1991) J. Cell Biol. 115, 1585-1594). We applied this approach to study the interactions of the H1 and H2 human asialoglycoprotein receptor subunits with coated pits. The lateral mobilities of singly expressed and coexpressed H1 and H2B (the H2 species that reaches the cell surface) were measured by fluorescence photobleaching recovery. They were compared with mutant proteins, H1(5A) (Tyr-5 replaced by Ala) and H2(5A) (Phe-5 replaced by Ala). While the mobile fraction of H1, H2B, and their mutants were similar, the lateral diffusion rate (measured by D, the lateral diffusion coefficient) was significantly slower for H1, whether expressed alone orwith H2B. Coexpression with H1 reduced D of H2B to that of H1. Disruption of the clathrin lattices by hypertonic medium elevated D of H1, H1(5A), H2B, and H2(5A) to the same final level, without affecting their mobile fractions. Cytosol acidification, which retains altered clathrin lattices attached to the membrane and prevents coated vesicle formation, immobilized part of the H1 molecules, reflecting stable entrapment in ''frozen'' coated pits. H1(5A), H2B, and H2(5A) were not affected: however, coexpression of H2B with H1 conferred the sensitivity to cytosol acidification on H2B. Our results suggest that H1 lateral mobility is inhibited by dynamic interactions with coated pits in which Tyr-5 is involved. H2B resembles H1(5A) rather than H1, and its interactions with coated pits are weaker; efficient interaction of H2B with coated pits depends on complex formation with H1.

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Documento generato il 23/01/20 alle ore 18:28:01