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Titolo:
COMPLEXITY OF IL-1-BETA INDUCED GENE-EXPRESSION PATTERN IN HUMAN ARTICULAR CHONDROCYTES
Autore:
MARGERIE D; FLECHTENMACHER J; BUTTNER FH; KARBOWSKI A; PUHL W; SCHLEYERBACH R; BARTNIK E;
Indirizzi:
HOECHST MARION ROUSSEL,H528,RHEINGAUSTR 190 D-65174 WIESBADEN GERMANY HOECHST MARION ROUSSEL D-65174 WIESBADEN GERMANY
Titolo Testata:
Osteoarthritis and cartilage
fascicolo: 2, volume: 5, anno: 1997,
pagine: 129 - 138
SICI:
1063-4584(1997)5:2<129:COIIGP>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARTILAGE PROTEOGLYCAN SYNTHESIS; SYNOVIAL-FLUID; MESSENGER-RNA; NITRIC-OXIDE; FIBRONECTIN FRAGMENTS; DIFFERENTIAL DISPLAY; OSTEOARTHRITIC CARTILAGE; RHEUMATOID-ARTHRITIS; EXCISION-REPAIR; INTERLEUKIN-1;
Keywords:
DIFFERENTIAL DISPLAY; CHONDROCYTES; IL-1; OSTEOARTHRITIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
D. Margerie et al., "COMPLEXITY OF IL-1-BETA INDUCED GENE-EXPRESSION PATTERN IN HUMAN ARTICULAR CHONDROCYTES", Osteoarthritis and cartilage, 5(2), 1997, pp. 129-138

Abstract

The mRNA fingerprinting technique, differential display reverse transcription polymerase chain (DDRT-PCR), was used to detect changes in the overall pattern of gene expression in human articular knee chondrocytes induced by interleulrin-1 beta (IL-1 beta), the prototypical inducer of catabolic responses in degenerate joint diseases. One hundred different primer combinations generated approximately 10 000 different PCR fragments for IL-1 beta treated, as well as for untreated human chondrocytes, cultivated in alginate beads. This represented 53% of all expressed chondrocyte genes as based on statistical considerations. Side by side comparisons of differential display patterns originating from two different donor tissues yielded 44 reproducibly, differentially-displayed cDNA fragments, which were subcloned and sequenced. Sequencehomology searches revealed sequence identities to the human necrosis factor alpha (TNF-alpha) and IL-1 regulated gene TSG-6, fibronectin, osteopontin, calnexin, and the DNA repair enzyme ERCC5. The differential expression was confirmed with Northern and quantitative PCR analyses. The known function of these genes and their known IL-1 responsiveness indicate that the employed model system reflects the pleiotropic effects of IL-1 on the overall gene expression in human articular chondrocytes and identifies genes involved in very different biochemical pathways. Twenty-seven cDNAs lacked sequence homologies to known genes andmay represent novel genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 05:40:46