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Titolo:
AN IN-VITRO VIDEOMICROSCOPIC TEST SYSTEM TO ASSESS PUTATIVE FILARICIDAL AGENTS
Autore:
ESCOBAR GY; BERNAS MJ; CASE TC; WITT B; HARTLINE J; WAY DL; WITTE MH; BORGS P; WITTE CL;
Indirizzi:
UNIV ARIZONA,DEPT SURG TUCSON AZ 00000
Titolo Testata:
Lymphology
, volume: 27, anno: 1994, supplemento:, S
pagine: 377 - 379
SICI:
0024-7766(1994)27:<377:AIVTST>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRUGIA-MALAYI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
3
Recensione:
Indirizzi per estratti:
Citazione:
G.Y. Escobar et al., "AN IN-VITRO VIDEOMICROSCOPIC TEST SYSTEM TO ASSESS PUTATIVE FILARICIDAL AGENTS", Lymphology, 27, 1994, pp. 377-379

Abstract

In an extension of our earlier in vivo and in vitro videomicroscopic studies of the Brugia malayi life cycle in ferrets, we examined the effect of putative filaricidal agents on adult worm motility and survival. Two to twelve months after 75 L(3) stage B. malayi larvae were injected into each groin, ferrets displayed numerous dilated and incompetent hindlimb lymphatics harboring nests of adult nematodes. After segmental lymphatic ligation (distal and proximal to worm nests) and excision, adult worms were allowed to exit the incised lymphatic segment into tissue culture media. These worms typically survived in vitro for 3-12 weeks. Adult worms were placed in a viewing plastic well and using videomicroscopy individual worm motility and survival was monitored against putative filaricidal agents as graphically depicted on a video. Preliminary findings support the potential value of this model to simulate the parasite's intralymphatic environment and assess filaricidal activity in an analogous manner to performing in vitro sensitivity testing of bacteria to antibiotic drugs.

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Documento generato il 01/12/20 alle ore 19:21:38