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Titolo:
LOCAL-ACTION OF PHOSPHATE-DEPLETION AND INSULIN-LIKE GROWTH-FACTOR-1 ON IN-VITRO PRODUCTION OF 1,25-DIHYDROXYVITAMIN-D BY CULTURED MAMMALIAN KIDNEY-CELLS
Autore:
CONDAMINE L; VZTOVSNIK F; FRIEDLANDER G; MENAA C; GARABEDIAN M;
Indirizzi:
HOP ST VINCENT DE PAUL,CNRS,URA 583,AVE DENFERT ROCHEREAU F-75014 PARIS FRANCE HOP ST VINCENT DE PAUL,CNRS,URA 583 F-75014 PARIS FRANCE FAC XAVIER BICHAT,INSERM,U251 F-75018 PARIS FRANCE
Titolo Testata:
The Journal of clinical investigation
fascicolo: 4, volume: 94, anno: 1994,
pagine: 1673 - 1679
SICI:
0021-9738(1994)94:4<1673:LOPAIG>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SODIUM-COUPLED UPTAKES; PROXIMAL TUBULAR CELLS; VITAMIN-D METABOLITES; FACTOR-I; 25-HYDROXYCHOLECALCIFEROL METABOLISM; 24,25-DIHYDROXYVITAMIN-D3 PRODUCTION; SERUM 1,25-DIHYDROXYVITAMIN-D; DIETARY PHOSPHORUS; EPITHELIAL-CELLS; PI TRANSPORT;
Keywords:
CALCITRIOL; OK CELLS; LLC-PK 1 CELLS; RABBITS PROXIMAL TUBULAR CELLS; SOMATOMEDIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
L. Condamine et al., "LOCAL-ACTION OF PHOSPHATE-DEPLETION AND INSULIN-LIKE GROWTH-FACTOR-1 ON IN-VITRO PRODUCTION OF 1,25-DIHYDROXYVITAMIN-D BY CULTURED MAMMALIAN KIDNEY-CELLS", The Journal of clinical investigation, 94(4), 1994, pp. 1673-1679

Abstract

The hormonal form of vitamin D, 1,25(OH)(2)D, is synthesized mostly in proximal renal tubular cells. Experimental and clinical studies suggest that the growth hormone may be involved in growth-related fluctuations of plasma 1,25(OH)(2)D and in the increase of 1,25(OH)(2)D induced by in vivo phosphate deprivation, an action possibly mediated by insulin-like growth factor 1 (IGF 1). We tested the effects of phosphate depletion and IGF 1 addition on 1,25(OH)(2)D-3 production in cultured kidney cells: opossum kidney (OK) cells, LLC-PK 1, and rabbit's proximal tubular cells. Confluent cell monolayers were preincubated in various phosphate concentrations, in the presence and absence of IGF 1, Then, 5 nM of [H-3]25(OH)D-3 or 2 mu M of 25(OH)D-3 were added to the medium and the cells were incubated for a further 120 min. The amount of biosynthesized 1,25(OH)(2)D-3 in lipid extracts was determined after two different straight phase high performance liquid chromatographies. The experiment showed the following: (a) LLC-PK 1 and rabbit's cells expressed a detectable ability to synthesize 1,25(OH)(2)D-3, while OK cells did not. (b) Partial or total phosphate deprivation increased theamount of 1,25(OH)(2)D-3 produced, respectively in LLC-PK 1 and in rabbit's cells. (c) IGF 1 (25 ng/ml) increased 1,25(OH)(2)D-3 productionin rabbit's cells, particularly in phosphate-free medium (1.6-fold), and in LLC-PK 1 cells, in partial phosphate depletion (2.75-fold in 1 mM phosphate, P = 0.015, n = 5, and 3.2-fold in 0.5 mM phosphate, P = 0.043, n = 4). Our findings demonstrate a local action of phosphate depletion and of IGF 1 on 1,25-dihydroxyvitamin D-3 production

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 09:49:13