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Titolo:
EFFECTS OF TOLUIDINE BLUE AND DESTAINING REAGENTS USED IN SEXUAL ASSAULT EXAMINATIONS ON THE ABILITY TO OBTAIN DNA PROFILES FROM POSTCOITALVAGINAL SWABS
Autore:
HOCHMEISTER MN; WHELAN M; BORER UV; GEHRIG C; BINDA S; BERZLANOVICH A; RAUCH E; DIRNHOFER R;
Indirizzi:
UNIV BERN,INST LEGAL MED,BUEHLSTR 20 CH-3012 BERN SWITZERLAND POMERADO HOSP POWAY CA 00000 UNIV VIENNA,INST LEGAL MED VIENNA AUSTRIA UNIV MUNICH,INST LEGAL MED MUNICH GERMANY
Titolo Testata:
Journal of forensic sciences
fascicolo: 2, volume: 42, anno: 1997,
pagine: 316 - 319
SICI:
0022-1198(1997)42:2<316:EOTBAD>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPERM; LUBRICANT; RAPE;
Keywords:
FORENSIC SCIENCE; SEXUAL ASSAULT; VAGINAL SWABS; PERINEAL LACERATIONS; TOLUIDINE BLUE; ACETIC ACID; LUBRICANTS; DNA; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; POLYMERASE CHAIN REACTION; GENETIC TYPING; D2S44; D4S139; D5S110; D10S28; TH01; TPOX; CSF1PO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
M.N. Hochmeister et al., "EFFECTS OF TOLUIDINE BLUE AND DESTAINING REAGENTS USED IN SEXUAL ASSAULT EXAMINATIONS ON THE ABILITY TO OBTAIN DNA PROFILES FROM POSTCOITALVAGINAL SWABS", Journal of forensic sciences, 42(2), 1997, pp. 316-319

Abstract

Toluidine blue is an important tool to detect and document genital and perianal injuries following sexual assault. Application of toluidineblue dye and its subsequent removal from unstained areas by means of a destaining reagent, such as diluted acetic acid or a lubricant has been shown to increase the detection rate of posterior fourchette lacerations from 16% to 40% in adult rape victims. Currently, limited information on toluidine blue positive findings in sexually active control groups imposes some limitation on the interpretation of these injuries. Because injuries could otherwise be attributed to improper handling of an examination speculum or the improper insertion of the examining finger, the toluidine blue test should be performed prior to any digital or speculum examination and thus prior to the collection of forensic evidence. For forensic DNA identity testing, it becomes pertinent todetermine whether toluidine blue and the destaining reagents used in a sexual assault examination have an adverse effect on the recovery ofhigh molecular weight DNA from postcoital vaginal swabs and thereby have an impact on restriction fragment length polymorphism (RFLP) analysis or PCR-based tests. It is known that some of the lubricants used can have a destructive effect on sperm motility. In order to investigate the potential effects, postcoital vaginal swabs were taken 6 h aftersexual intercourse and exposed directly to 1% toluidine blue in aqueous solution, 1-10% acetic acid, and various surgical and vaginal lubricants. Subsequently, the DNA was isolated and DNA identity typing (RFLP and PCR-based) was performed. The results demonstrate, that these reagents have no negative effect on the ability to obtain DNA profiles, either RFLP or PCR-based, from shallow and deep vaginal swabs. The quantity and quality of extractable high molecular weight DNA obtained was comparable with that from uncontaminated postcoital vaginal swabs. RFLP patterns and PCR-based typing results on the D1S80, HUMTH01, TPOX,and CSF1PO loci were consistent with the uncontaminated control swabsand the corresponding whole blood samples of the donors. Therefore, evidentiary material inadvertently contaminated with these reagents canbe successfully typed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 22:27:40