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Titolo:
HUMAN METABOTROPIC GLUTAMATE-RECEPTOR-1 - MESSENGER-RNA DISTRIBUTION,CHROMOSOME LOCALIZATION AND FUNCTIONAL EXPRESSION OF 2 SPLICE VARIANTS
Autore:
STEPHAN D; BON C; HOLZWARTH JA; GALVAN M; PRUSS RM;
Indirizzi:
SYNTHELABO BIOMOL,16 RUE ANKARA F-67080 STRASBOURG FRANCE SYNTHELABO BIOMOL F-67080 STRASBOURG FRANCE
Titolo Testata:
Neuropharmacology
fascicolo: 12, volume: 35, anno: 1996,
pagine: 1649 - 1660
SICI:
0028-3908(1996)35:12<1649:HMG-MD>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; MGLUR1 MUTANT MICE; KIDNEY CELL-LINE; PHARMACOLOGICAL CHARACTERIZATION; MOLECULAR-CLONING; BRAIN-FUNCTION; RAT; SUBTYPES; AGONIST; DERIVATIVES;
Keywords:
METABOTROPIC GLUTAMATE RECEPTOR; CALCIUM IMAGING; HUMAN CHROMOSOME 6; XENOPUS OOCYTE ELECTROPHYSIOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
D. Stephan et al., "HUMAN METABOTROPIC GLUTAMATE-RECEPTOR-1 - MESSENGER-RNA DISTRIBUTION,CHROMOSOME LOCALIZATION AND FUNCTIONAL EXPRESSION OF 2 SPLICE VARIANTS", Neuropharmacology, 35(12), 1996, pp. 1649-1660

Abstract

We have isolated clones from a human brain cDNA library coding for two splice variants of mGluR1. The combined sequences account for 6864 base pairs (bp) of the similar to 7 kilobase (kb) transcript seen on northern blots of human brain mRNA. The distribution of mGluR1 mRNA in human brain is similar to that found in rat brain and the gene for mGluR1 is localized on human chromosome 6. The mRNA for mGluRl alpha contains an open reading frame that codes for a 1194 amino acid protein, which is slightly smaller than rat mGluR1 alpha. The major structural difference noted between the human and rat mGluR1 is a deletion of 21 nucleotides which would result in the loss of seven amino acids in the middle of a proline- and glutamine-rich region of the C-terminal tail of mGluR1 alpha. The 85 bp exon that results in the HmGluR1 beta splicevariant was found to code for a protein with two amino acid differences compared to the rat receptor. Functional and pharmacological characterization of heterologously expressed human mGluR1 was performed using electrophysiological recordings from Xenopus oocytes and calcium imaging in HEK-293 cells. No major differences were found in the responseof human mGluR1 to typical agonists and antagonists compared to the rat, or in the behavior of the two splice variants. (C) 1997 Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:15:02