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Titolo:
EFFECT OF GRANULOCYTE-COLONY-STIMULATING FACTOR ON PIG-BLOOD PLATELETS TREATED WITH CISPLATIN IN-VITRO
Autore:
OLAS B; WACHOWICZ B; WACHOWICZ N;
Indirizzi:
UNIV LODZ,DEPT GEN BIOCHEM,INST BIOCHEM,BANACHA 12-16 PL-90237 LODZ POLAND UNIV LODZ,DEPT GEN BIOCHEM,INST BIOCHEM PL-90237 LODZ POLAND
Titolo Testata:
Anti-cancer drugs
fascicolo: 2, volume: 8, anno: 1997,
pagine: 204 - 209
SICI:
0959-4973(1997)8:2<204:EOGFOP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATION; NEUTROPHIL; CHEMOTHERAPY; CANCER; CELLS;
Keywords:
BLOOD PLATELETS; CISPLATIN; G-CSF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
B. Olas et al., "EFFECT OF GRANULOCYTE-COLONY-STIMULATING FACTOR ON PIG-BLOOD PLATELETS TREATED WITH CISPLATIN IN-VITRO", Anti-cancer drugs, 8(2), 1997, pp. 204-209

Abstract

The effects of granulocyte colony stimulating factor (G-CSF) and cisplatin in vitro on the peroxidation of pig platelet endogenous arachidonate were investigated. Estimation of the level of malonyldialdehyde (MDA) after stimulation of platelets with thrombin served as an indicator of this process. MDA concentration was determined by means of a modified method with thiobarbituric acid. The effects of G-CSF on thrombin-induced formation of MDA in platelets was dependent on the concentration and the time of platelet preincubation with this cytokine. In theplatelets obtained from the whole blood preincubated with the highestconcentration of G-CSF (8 mu g/ml), a significant increase of MDA formation was observed (p<0.01). Cisplatin (20 mu M) had a strong inhibitory effect on arachidonic acid metabolism in platelets (p<0.001). In the presence of G-CSF (0.8 mu g/ml) in incubation medium the inhibitoryeffect of cisplatin on platelet arachidonate pathway was reduced. Theresults showed that G-CSF had a protective effect against cisplatin action on platelets.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 21:30:19