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Titolo:
PRODUCTION OF MONOCLONAL-ANTIBODIES RECOGNIZING CANCER-ASSOCIATED ANTIGENS EXPRESSED ON MUCIN-TYPE SUGAR CHAINS
Autore:
KUROSAKA A; IKEDA K; SAKURAGI N; FUJIMOTO S;
Indirizzi:
HOKKAIDO UNIV,SCH MED,DEPT OBSTET & GYNECOL,N15,W7 SAPPORO 060 JAPAN KYOTO SANGYO UNIV,FAC ENGN,DEPT BIOTECHNOL,KITA KU KYOTO 603 JAPAN
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 3, volume: 203, anno: 1994,
pagine: 1828 - 1834
SICI:
0006-291X(1994)203:3<1828:POMRCA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN RECTAL ADENOCARCINOMA; BOVINE SUBMAXILLARY MUCIN; GLYCOPROTEINS; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
A. Kurosaka et al., "PRODUCTION OF MONOCLONAL-ANTIBODIES RECOGNIZING CANCER-ASSOCIATED ANTIGENS EXPRESSED ON MUCIN-TYPE SUGAR CHAINS", Biochemical and biophysical research communications, 203(3), 1994, pp. 1828-1834

Abstract

To obtain monoclonal antibodies directed to mucin-type sugar chains, mice were immunized with bovine submaxillary mucin (BSM) that had beenconjugated with ovalbumin. Conjugation of BSM with ovalbumin enhancedthe antigenicity of BSM to about five to ten times that of intact BSMand resulted in the establishment of ten hybridomas, all of which secreted monoclonal antibodies toward BSM. Most of the antibodies secreted by these hybridomas did not react with glycolipids but did react with glycoproteins. Several antibodies lost their reactivity when sialic acid residues were removed from BSM, indicating that these antibodies recognize carbohydrate moieties of mucins. Immunohistochemical studiesrevealed that three of the antibodies recognized human ovarian cancer-associated carbohydrate antigens. In addition, one of these three antibodies reacted with a human cultured colonic cancer cell line. The protocol described in this paper was effective in producing monoclonal antibodies that recognize mucin-carbohydrates and some of the generatedantibodies can be applied to the detection of cancers. (C) 1994 Academic Press. Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:33:59