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Titolo:
CHARACTERIZATION OF RIFAMPIN RESISTANCE IN PATHOGENIC MYCOBACTERIA
Autore:
WILLIAMS DL; WAGUESPACK C; EISENACH K; CRAWFORD JT; PORTAELS F; SALFINGER M; NOLAN CM; ABE C; STICHTGROH V; GILLIS TP;
Indirizzi:
GWL,HANSENS DIS CTR,RES BRANCH LAB,HANSENS DIS RES LAB,POB 25072 BATON ROUGE LA 70894 VA MED HOSP LITTLE ROCK AR 00000 CTR DIS CONTROL & PREVENT ATLANTA GA 30341 INST TROP MED PRINCE LEOPOLD B-2000 ANTWERP BELGIUM NEW YORK STATE DEPT HLTH ALBANY NY 12201 SEATTLE KING CTY DEPT PUBL HLTH SEATTLE WA 00000 RES INST TB TOKYO JAPAN ARMAUER HANSEN INST WURZBURG GERMANY
Titolo Testata:
Antimicrobial agents and chemotherapy
fascicolo: 10, volume: 38, anno: 1994,
pagine: 2380 - 2386
SICI:
0066-4804(1994)38:10<2380:CORRIP>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXOGENOUS REINFECTION; ESCHERICHIA-COLI; TUBERCULOSIS; LEPRAE; DNA; MUTATIONS; GENE; POLYMORPHISMS; POLYMERASE; EMERGENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
D.L. Williams et al., "CHARACTERIZATION OF RIFAMPIN RESISTANCE IN PATHOGENIC MYCOBACTERIA", Antimicrobial agents and chemotherapy, 38(10), 1994, pp. 2380-2386

Abstract

The emergence of rifampin-resistant strains of pathogenic mycobacteria has threatened the usefulness of this drug in treating mycobacterialdiseases. Critical to the treatment of individuals infected with resistant strains is the rapid identification of these strains directly from clinical specimens. It has been shown that resistance to rifampin in Mycobacterium tuberculosis and Mycobacterium leprae apparently involves mutations in the rpoB gene encoding the beta-subunit of the RNA polymerases of these species. DNA sequences were obtained from a 305-bp fragment of the rpoB gene from 110 rifampin-resistant and 10 rifampin-susceptible strains of M. tuberculosis from diverse geographical regions throughout the world. In 102 of 110 rifampin-resistant strains 16 mutations affecting 13 amino acids were observed. No mutations were observed in rifampin-susceptible strains. No association was found between particular mutations in the rpoB gene and drug susceptibility patterns of multidrug-resistant M. tuberculosis strains. Drug-resistant M. tuberculosis strains from the same outbreak and exhibiting the same IS6110 DNA fingerprint and drug susceptibility pattern contained the samemutation in the rpoB gene. However, mutations are not correlated withIS6110 profiling outside of epidemics. The evolution of rifampin resistance as a consequence of mutations in the rpoB gene was documented in a patient who developed rifampin resistance during the course of treatment. Rifampin-resistant strains of M. leprae, Mycobacterium avium, and Mycobacterium africanum contained mutations in the rpoB gene similar to that documented for M. tuberculosis. This information served as the basis for developing a rapid DNA diagnostic assay (PCR-heteroduplex formation) for the detection of rifampin susceptibility of M. tuberculosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 11:23:49