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Titolo:
ANALYSIS OF THE GENOME OF VIBRIO-COSTICOLA - PULSED-FIELD GEL-ELECTROPHORETIC ANALYSIS OF GENOME SIZE AND PLASMID CONTENT
Autore:
MELLADO E; GARCIA MT; NIETO JJ; KAPLAN S; VENTOSA A;
Indirizzi:
UNIV SEVILLA,FAC PHARM,DEPT MICROBIOL & PARASITOL E-41012 SEVILLE SPAIN UNIV SEVILLA,FAC PHARM,DEPT MICROBIOL & PARASITOL E-41012 SEVILLE SPAIN UNIV TEXAS,SCH MED,DEPT MICROBIOL & MOL GENET HOUSTON TX 00000
Titolo Testata:
Systematic and applied microbiology
fascicolo: 1, volume: 20, anno: 1997,
pagine: 20 - 26
SICI:
0723-2020(1997)20:1<20:AOTGOV>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MODERATELY HALOPHILIC EUBACTERIA; HALOFERAX-MEDITERRANEI; ESCHERICHIA-COLI; DNA-MOLECULES; DAM; METHYLATION; SEPARATION;
Keywords:
VIBRIO COSTICOLA; MODERATE HALOPHILES; PULSED FIELD GEL ELECTROPHORESIS; GENOME SIZE; PLASMID CHARACTERIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
E. Mellado et al., "ANALYSIS OF THE GENOME OF VIBRIO-COSTICOLA - PULSED-FIELD GEL-ELECTROPHORETIC ANALYSIS OF GENOME SIZE AND PLASMID CONTENT", Systematic and applied microbiology, 20(1), 1997, pp. 20-26

Abstract

The genome organization of the moderate halophile Vibrio costicola has been investigated. To estimate its genome size, large restriction fragments of genomic DNA were separated by pulsed-field gel electrophoresis (PFGE) after digestion with restriction endonucleases. Enzymes SfiI and MboI generated 25 and 6 restriction fragments, respectively. Thegenome size of V. costicola E-367 was estimated to be 2,505 kb (SfiI)or 2,259 kb (MboI). Genomic DNA digested with SfiI also permitted thecalculation of the genome sizes of five additional V. costicola strains, which ranged from 2,100 to 2,600 kb. Besides, three plasmids, designated pVC1, pVC2 and pVC3 (of 2.95 kb, 19 kb and 21 kb, respectively), as well as a megaplasmid, were detected in V. costicola E-367. The restriction map of pVC1 was determined following the use of 14 restriction enzymes, showing cleavage sites for BamHI, BglII, HindII and SalI. Plasmid pVC1 is a candidate for use in the development of cloning vectors suitable for genetic manipulation of V: costicola. An interestingfinding is that the DNA of V. costicola is likely to be highly methylated because digestion with MboI yields few cleavage sites. Additionally, different MboI restriction patterns were observed when this moderate halophile was grown at different salinities. This suggests that themethylation system of this halophile may be affected by the salinity of the growth medium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:23:02