Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CHARACTERIZATION OF HETERORHABDITIS ISOLATES BY PCR AMPLIFICATION OF SEGMENTS OF MTDNA AND RDNA GENES
Autore:
JOYCE SA; BURNELL AM; POWERS TO;
Indirizzi:
ST PATRICKS COLL,DEPT BIOL MAYNOOTH KILDARE IRELAND UNIV NEBRASKA,DEPT PLANT PATHOL LINCOLN NE 68583
Titolo Testata:
Journal of nematology
fascicolo: 3, volume: 26, anno: 1994,
pagine: 260 - 270
SICI:
0022-300X(1994)26:3<260:COHIBP>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RHABDITIDA; NEMATODA; GENUS;
Keywords:
ENTOMOPATHOGENIC NEMATODE; HETERORHABDITIS; MOLECULAR DIAGNOSTICS; MTDNA; NEMATODE; RIBOSOMAL DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
S.A. Joyce et al., "CHARACTERIZATION OF HETERORHABDITIS ISOLATES BY PCR AMPLIFICATION OF SEGMENTS OF MTDNA AND RDNA GENES", Journal of nematology, 26(3), 1994, pp. 260-270

Abstract

Restriction digests of amplified DNA from the mitochondrial genome and the nuclear ribosomal internally transcribed spacer region have beenevaluated as genetic markers for species groups in Heterorhabditis. Six RFLP profiles have been identified. These profiles supported groupings determined by cross-breeding studies and were in agreement with less definitive groupings based on other biochemical and molecular methods. Digestion patterns of both amplification products provided strong evidence for the recognition of species groups, which include Irish, NW European, tropical, and a H. bacteriophora complex. The H. bacteriophora complex could be further resolved into three genotypes represented by H. zealandica, the H. bacteriophora, Brecon (Australian) type isolate for H. bacteriophora, and a grouping composed of isolates NC1, V16, H182, and HP88. All cultures obtained of the H. megidis isolate were identical to the NW European group. These results could be used to aid monitoring of field release of Heterorhabditis as well as allowing a rapid initial assessment of taxonomic grouping.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 05:42:40