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Titolo:
IDENTIFICATION OF GENES OVEREXPRESSED IN TUMORS THROUGH PREFERENTIAL EXPRESSION SCREENING IN TROPHOBLASTS
Autore:
CHASSIN D; BENIFLA JL; DELATTRE C; FERNANDEZ H; GINISTY D; JANNEAU JL; PRADE M; CONTESSO G; CAILLOU B; TOURNAIRE M; FRYDMAN R; ELIAS D; BEDOSSA P; BIDART JM; BELLET D; KOMAN A;
Indirizzi:
UNIV PARIS 05,FAC SCI PHARMACEUT & BIOL,IMMUNOL TUMEURS LAB,CNRS,URA 1484,4 AVE OBSERV F-75006 PARIS FRANCE UNIV PARIS 05,FAC SCI PHARMACEUT & BIOL,IMMUNOL TUMEURS LAB,CNRS,URA 1484 F-75006 PARIS FRANCE HOP BICHAT F-75018 PARIS FRANCE INST GUSTAVE ROUSSY,SERV IMMUNOL MOLEC F-94805 VILLEJUIF FRANCE HOP ANTOINE BECLERE F-92140 CLAMART FRANCE INST GUSTAVE ROUSSY,SERV ANAT PATHOL B F-94805 VILLEJUIF FRANCE INST GUSTAVE ROUSSY,SERV ANAT PATHOL A F-94805 VILLEJUIF FRANCE HOP ST VINCENT DE PAUL F-75014 PARIS FRANCE INST GUSTAVE ROUSSY,SERV CHIRURG F-94805 VILLEJUIF FRANCE HOP KREMLIN BICETRE,SERV ANAT PATHOL F-94270 LE KREMLIN BICETR FRANCE
Titolo Testata:
Cancer research
fascicolo: 19, volume: 54, anno: 1994,
pagine: 5217 - 5223
SICI:
0008-5472(1994)54:19<5217:IOGOIT>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED RAT HEPATOMAS; RIBOSOMAL-PROTEIN; HUMAN-PLACENTA; GROWTH-FACTOR; ONCOGENES; CELLS; DIFFERENTIATION; TRANSCRIPT; RECEPTOR; DECIDUA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
D. Chassin et al., "IDENTIFICATION OF GENES OVEREXPRESSED IN TUMORS THROUGH PREFERENTIAL EXPRESSION SCREENING IN TROPHOBLASTS", Cancer research, 54(19), 1994, pp. 5217-5223

Abstract

Early trophoblastic cells share several features with neoplastic cells. Based on that observation, we attempted to identify genes overexpressed in tumors by analyzing genes preferentially expressed in trophoblasts. A subtracted library enriched in complementary DNA from early cytotrophoblasts was constructed, and the expression level of selected recombinants nas analyzed on a large panel of normal and tumor tissues. The library was prepared using a polymerase chain reaction-based complementary DNA subtraction method with 6-week amenorrhea cytotrophoblast endoplasmic reticulum-bound RNA as target, and a mixture of complementary DNA prepared from terminal placenta and activated T-lymphocytes as driver. Two rounds of screening were performed to isolate clones preferentially expressed in early placenta. From a total number of recombinant clones estimated at 32,000 in the subtracted library, 594 inserts were analyzed by Southern blot and 21 sequences were isolated as corresponding to genes highly expressed in early placenta. Eleven encoded known molecules, such as carcinoembryonic antigen, human chorionic gonadotropin, and mitochondrial rRNAs. Ten sequences represented novel genes. Northern blot analysis confirmed that most of these genes were preferentially expressed in early trophoblast in comparison to terminal placenta. Three clones that gave detectable hybridization signals ontotal RNA were extensively studied and were found to be overexpressedin various tumors. Two of these clones, designated B9 and E4, were later identified as corresponding to genes coding for the putative ribosomal protein S18 and the bifunctional enzyme ADE2H1 involved in purinebiosynthesis, respectively. Expression of the third done, E9, was increased up to 10-fold in breast cancer tissues in comparison with normal counterparts. Present results confirm that many genes expressed in the trophoblast are overexpressed in malignant cells. This approach could provide a general targeted method for the identification of genes overexpressed in various neoplastic cell types.

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Documento generato il 05/12/20 alle ore 23:58:20