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Titolo:
RAB7 - NMR AND KINETICS ANALYSIS OF INTACT AND C-TERMINAL TRUNCATED CONSTRUCTS
Autore:
NEU M; BRACHVOGEL V; OSCHKINAT H; ZERIAL M; METCALF P;
Indirizzi:
EUROPEAN MOL BIOL LAB,POSTFACH 102209 MEYERHOFSTR 1 D-69012 HEIDELBERG GERMANY EUROPEAN MOL BIOL LAB D-69012 HEIDELBERG GERMANY
Titolo Testata:
Proteins
fascicolo: 2, volume: 27, anno: 1997,
pagine: 204 - 209
SICI:
0887-3585(1997)27:2<204:R-NAKA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTP-BINDING-PROTEINS; GDP-DISSOCIATION INHIBITOR; NUCLEOTIDE EXCHANGE; TRANSPORT; SPECIFICITY; DOMAIN; SIGNAL; SWITCH; FORMS; YPT1;
Keywords:
RAB7; GTPASE; VESICLE; TARGETING; CRYSTAL; KINETICS; NMR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
M. Neu et al., "RAB7 - NMR AND KINETICS ANALYSIS OF INTACT AND C-TERMINAL TRUNCATED CONSTRUCTS", Proteins, 27(2), 1997, pp. 204-209

Abstract

Rab proteins are a family of similar to 25kD ras-related GTPases which are associated with distinct intracellular membranes where they control vesicle traffic between intracellular compartments, The late-endosomal rab protein rab7(1-207), (lacking only the C-terminal lipids of the native molecule) and three C-terminal truncated constructs rab7(1-202), rab7(1-197) and rab7(1-182) were purified using an E. coli expression system, The C-terminal tail region of rab proteins is of special interest because it is thought to target rab proteins to particular intracellular membranes, A comparison of TOCSY-NMR spectra from intact rab7(1-207) and the tail-less construct rab7(1-182) suggested that muchof the C-terminal tail is flexible in solution. The GTP hydrolysis, and GDP association and dissociation rates for all the truncated and intact constructs were similar, showing that the tail region of rab7(1-207) has Little influence on the hydrolysis and exchange rates of the nucleotide. (C) 1997 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 11:13:31