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Titolo:
DETERMINATION OF CONJUGATED AND UNCONJUGATED SERUM 3-ALPHA-OH BILE-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
Autore:
GOLDSMITH RF; GRUCA M; OHALLORAN MT; EARL JW; GASKIN K;
Indirizzi:
CHILDRENS HOSP,JAMES FAIRFAX INST CLIN NUTR SYDNEY NSW 2050 AUSTRALIA CHILDRENS HOSP,DEPT BIOCHEM SYDNEY NSW 2050 AUSTRALIA
Titolo Testata:
Annals of clinical biochemistry
, volume: 31, anno: 1994,
parte:, 5
pagine: 479 - 484
SICI:
0004-5632(1994)31:<479:DOCAUS>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRELABELING REAGENT; FLUORESCENCE;
Keywords:
SOLID PHASE EXTRACTION; DERIVATIZATION; KETO BILE ACIDS; ENZYMATIC REDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
R.F. Goldsmith et al., "DETERMINATION OF CONJUGATED AND UNCONJUGATED SERUM 3-ALPHA-OH BILE-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY", Annals of clinical biochemistry, 31, 1994, pp. 479-484

Abstract

A Simple reverse-phase liquid chromatographic method has been developed for the quantitative measurement of 11 3 alpha-OH bile acids in paediatric serum samples. Bile acids are enzymatically reduced to the corresponding 3-keto compound and then derivatized with the fluorophore dansyl hydrazine. Preliminary fractionation of bile acids is not required. The limit of detection is 0.1 mu mol/L using a sample size of 200 mu L. One hundred and twenty-three serum samples were analysed by the high-performance liquid chromatography (HPLC) method and the results compared with a commercial kit method (Enzabile) presently used in manylaboratories for the measurement of total bile acids. There was a good correlation between the two methods (r = 0.96). The method is suitable for use in a clinical laboratory for the further investigation of those patients with abnormally high total bile acid levels where quantification of bile acid fractions is required.

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Documento generato il 07/07/20 alle ore 18:16:09