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Titolo:
EXPRESSION OF PREPROENKEPHALIN-A GENE AND PRESENCE OF MET-ENKEPHALIN IN DORSAL-ROOT GANGLIA OF THE ADULT-RAT
Autore:
POHL M; COLLIN E; BOURGOIN S; CONRATH M; BENOLIEL JJ; NEVO I; HAMON M; GIRAUD P; CESSELIN F;
Indirizzi:
UNIV PARIS 06,INSERM,U288,91 BLVD HOP F-75634 PARIS 13 FRANCE UNIV PARIS 06,INST NEUROSCI,CNRS,URA 1488 PARIS FRANCE FAC MED MARSEILLE,SECTEUR NORD,NEUROENDOCRINOL EXPTL LAB F-13385 MARSEILLE FRANCE
Titolo Testata:
Journal of neurochemistry
fascicolo: 4, volume: 63, anno: 1994,
pagine: 1226 - 1234
SICI:
0022-3042(1994)63:4<1226:EOPGAP>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMARY AFFERENT-FIBERS; MAMMALIAN SPINAL-CORD; MESSENGER-RNA; IMMUNOREACTIVE DYNORPHIN; SUBSTANCE-P; BETA-PREPROTACHYKININ; INSITU HYBRIDIZATION; INVITRO RELEASE; OPIOID-PEPTIDES; LOCALIZATION;
Keywords:
MET-ENKEPHALIN; RAT DORSAL ROOT GANGLIA; HPLC; POLYMERASE CHAIN REACTION; IN SITU HYBRIDIZATION; IMMUNOHISTOCHEMISTRY; NORTHERN BLOT; PREPROENKEPHALIN A;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
M. Pohl et al., "EXPRESSION OF PREPROENKEPHALIN-A GENE AND PRESENCE OF MET-ENKEPHALIN IN DORSAL-ROOT GANGLIA OF THE ADULT-RAT", Journal of neurochemistry, 63(4), 1994, pp. 1226-1234

Abstract

The expression of the preproenkephalin A gene was investigated in adult rat dorsal root ganglia (DRG). A radioimmunoassayable Met-enkephalin (ME)-like material was detected in 0.1 M HCl extracts of rat DRG, representing similar to 60 pg of ME equivalents/mg of protein. Chromatographic analyses indicated that the major component of the ME-like material coeluted with authentic ME. In northern blot experiments on totalRNA extracted from DRG, a cDNA probe corresponding to the entire coding region of rat preproenkaphalin A mRNA yielded a single band of the expected size for this mRNA, i.e., 1.5 kb. Polymerase chain reaction (PCR) experiments were carried out with DRG, striatum and DRG cDNAs generated a single band of 400 bp, as expected, whereas only trace amounts of this product were detectable using liver cDNAs. Nucleotide sequencing of the PCR product obtained with DRG cDNAs revealed a 100% homology with the 1,371-1,771 sequence of the preproenkephalin A gene. In situ hybridization with a cRNA probe showed that about 3.5% of DRG cellsexpressed the preproenkephalin A transcript. However, most of these cells probably did not process proenkephalin to enkephalins, as thorough immunohistochemical investigations with anti-ME antibodies allowed the detection of only one in similar to 6,000 cells (in 30 sections of DRG) that exhibited ME-like immunoreactivity. Cells expressing preproenkephalin A mRNA were intermediate-sized neurons, suggesting that primary afferent ME-containing fibers belong to the A category and may participate in a local (spinal) inhibitory control of nociception.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 02:38:41