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Titolo:
OCCURRENCE OF BACILLUS SPECIES ON EVERGREEN OAK LEAVES
Autore:
BRUNEL B; PERISSOL C; FERNANDEZ M; BOEUFGRAS JM; LEPETIT J;
Indirizzi:
ECOLE NATL SUPER AGRON MONTIPELLIER,1 PL PIERRE VIALA F-34060 MONTPELLIER 1 FRANCE UNIV SCI AIX MARSEILLE 3,FAC SCI & TECH ST JEROME,MICROBIOL LAB,SERV 452 F-13397 MARSEILLE 20 FRANCE UNIV LYON 1,ECOL MICROBIENNE SOL LAB,CNRS,URA 1450 F-69622 VILLEURBANNE FRANCE BIOMERIEUX API SYST,DEPT INFORMAT SCI F-38390 LA BALME GROTTES FRANCE
Titolo Testata:
FEMS microbiology, ecology
fascicolo: 4, volume: 14, anno: 1994,
pagine: 331 - 342
SICI:
0168-6496(1994)14:4<331:OOBSOE>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEOXYRIBONUCLEIC-ACID RELATEDNESS; 16S RIBOSOMAL-RNA; GENUS BACILLUS; NUMERICAL CLASSIFICATION; THURINGIENSIS; BACTERIAL; CEREUS; SOIL; IDENTIFICATION; MICROFLORA;
Keywords:
BACILLUS CEREUS; BACILLUS PUMILUS; EVERGREEN OAK LEAF; PHENOTYPIC CLASSIFICATION; DNA/DNA HYBRIDIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
B. Brunel et al., "OCCURRENCE OF BACILLUS SPECIES ON EVERGREEN OAK LEAVES", FEMS microbiology, ecology, 14(4), 1994, pp. 331-342

Abstract

Thirty-five spore-forming bacilli were isolated from evergreen oak leaves at four stages (one-year-old and two-year-old leaves, upper layerand underlayer litter leaves) and over the four seasons within one year. These isolates, plus five reference strains, were characterized morphologically and physiologically by a total of 100 tests and genetically by DNA/DNA hybridization. Phenotypic similarities of all strains were determined by numerical taxonomy, with the unweighted average linkage (UPGMA) algorithm and simple matching (S-SM) and Jaccard (S-J) coefficients used as measures for similarity. Three groups (A to C) were defined at a similarity level of 71% (S-SM) or 57% (S-J). They contained leaf isolates phenotypically related to Bacillus cereus, B. pumilusand B. circulans species respectively. The majority of the leaf isolates were assigned to B. cereus (34%) and B. pumilus (63%). DNA/DNA hybridization also discriminated three groups (genomic groups 1, 2 and unclassified strains) which presented a good correlation with the numerical analysis. Yet, DNA/DNA hybridization grouping revealed a higher degree of discrimination by defining four subgroups (1a, 1b, 2a and 2b). Genomic subgroup la contained leaf isolates belonging to the B. cereus species; isolates from genomic subgroup 1b belonged to B. mycoides species and isolates from genomic subgroup 2a belonged to B. pumilus species. Subgroup 2b consisted of a new genomic subspecies of B. pumiluswhich exhibited a degree of homology ranging from 53 to 64% with the B. pumilus type strain and a coefficient of divergence (Delta Tm) ranging from 5.5 to 7 degrees C. The different genomic groups presented different substrate metabolism capacities and a different spatial distribution on evergreen oak leaves. B. cereus strains (group 1) were predominantly located on litter leaves whereas B. pumilus strains (group 2)were found on the phyllosphere. In contrast with group 1, group 2 wasable to metabolize some sugars and pectin, while group 1 isolates were able to hydrolyze starch and glycogen. Thus, our hypothesis is that group 1 succeeds group 2 when the leaves are littered.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 07:11:33