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Titolo:
IMMUNOHISTOCHEMICAL STUDIES INDICATE MULTIPLE ENTEROENDOCRINE CELL-DIFFERENTIATION PATHWAYS IN THE MOUSE PROXIMAL SMALL-INTESTINE
Autore:
AIKEN KD; KISSLINGER JA; ROTH KA;
Indirizzi:
WASHINGTON UNIV,SCH MED,DEPT PATHOL,660 S EUCLID AVE,BOX 8118 ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT PATHOL ST LOUIS MO 63110
Titolo Testata:
Developmental dynamics
fascicolo: 1, volume: 201, anno: 1994,
pagine: 63 - 70
SICI:
1058-8388(1994)201:1<63:ISIMEC>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOCRINE-CELLS; TRANSGENIC MICE; SECRETIN-CELLS; SUBSTANCE-P; SEROTONIN; ORIGIN; GROWTH; POPULATIONS; EPITHELIUM; EXPRESSION;
Keywords:
GASTRIC INHIBITORY PEPTIDE; CRYPTS; VILLI; SECRETIN EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
K.D. Aiken et al., "IMMUNOHISTOCHEMICAL STUDIES INDICATE MULTIPLE ENTEROENDOCRINE CELL-DIFFERENTIATION PATHWAYS IN THE MOUSE PROXIMAL SMALL-INTESTINE", Developmental dynamics, 201(1), 1994, pp. 63-70

Abstract

The enteroendocrine cell system of the mammalian gastrointestinal tract is comprised of at least 16 different subpopulations. Each subpopulation shows a characteristic distribution along both the crypt-villus and cephalocaudal axes. In both the small intestine and colon of adultmice, multilabel immunohistochemistry has demonstrated that two or more neuroendocrine products can be coexpressed in various combinations in single cells along the crypt-villus axis, suggesting that enteroendocrine phenotypes may be actively regulated. Using bromodeoxyuridine (BrdU) incorporation and multilabel immunohistochemistry, we have previously demonstrated an enteroendocrine cell differentiation pathway consisting of two subpopulations of cells in the mouse proximal small intestine-one involving the sequential expression of substance P, serotonin, and secretin in cells migrating out of the crypts into the villi, and a second involving the expression of substance P and serotonin in cells which remain in the crypts. In this report, we use double label immunohistochemistry and BrdU incorporation to define the temporal andspatial interrelationships between gastrin, cholecystokinin (CCK), glucagon-like peptide-1 (GLP-1), and gastric inhibitory peptide (GIP) immunoreactive cells in the mouse proximal small intestine. The expression of these products was compared with that of substance P, serotonin,and secretin. Minimal overlap of expression was found in cells immunoreactive for substance P or serotonin with gastrin, CCK, GLP-1, or GIP; however, secretin was found colocalized in villus-associated gastrin, CCK, and GLP-1 containing cells. We demonstrate that, similar to thebidirectionally migrating substance P and serotonin expressing cells,gastrin, CCK, GLP-1, and secretin are expressed in upwardly migratingcells, and gastrin, CCK, and GLP-1 are expressed in downwardly migrating cells that fail to express secretin. GIP containing cells only rarely coexpressed any of the products examined, but were found both in the villi and the crypts, suggesting both upwardly and downwardly migrating populations. These findings demonstrate several novel enteroendocrine cell differentiation pathways. In addition, the expression of secretin in the villi, but not in the crypts, by two otherwise distinct differentiation pathways, and the lack of secretin expression by villus-associated G;IP expressing cells, suggests that local factors presentin the crypts and/or on the villi are necessary, but not sufficient, for secretin expression. (C) 1994 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 23:05:55