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Titolo:
OPTIMIZING THE MEASUREMENT OF REGIONAL CEREBRAL GLUCOSE CONSUMPTION WITH [6-C-14]GLUCOSE
Autore:
HAWKINS RA; HAWKINS PA; MANS AM; VINA JR; DEJOSEPH MR;
Indirizzi:
UNIV HLTH SCI CHICAGO MED SCH,DEPT PHYSIOL & BIOPHYS,3333 GREEN BAY RD N CHICAGO IL 60064
Titolo Testata:
Journal of neuroscience methods
fascicolo: 1, volume: 54, anno: 1994,
pagine: 49 - 62
SICI:
0165-0270(1994)54:1<49:OTMORC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
BICUCULLINE-INDUCED SEIZURES; INDUCED STATUS EPILEPTICUS; BLOOD-BRAIN-BARRIER; METABOLIC CHANGES; RATS; INVIVO; FLUORODEOXYGLUCOSE; CORTEX;
Keywords:
AUTORADIOGRAPHY; BRAIN GLUCOSE METABOLISM; GLUCOSE ISOTOPE; (RAT);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
R.A. Hawkins et al., "OPTIMIZING THE MEASUREMENT OF REGIONAL CEREBRAL GLUCOSE CONSUMPTION WITH [6-C-14]GLUCOSE", Journal of neuroscience methods, 54(1), 1994, pp. 49-62

Abstract

[6-C-14]Glucose is used to trace the cerebral metabolic rate of glucose (CMR(Glc)) in vivo in experiments lasting 5-10 min. Initially C-14 is trapped in intermediary metabolite pools. Subsequently C-14 is lostas a function of time and metabolic rate, primarily as (CO2)-C-14. Experiments were designed to evaluate the rate of C-14 lost as (CO2)-C-14 Or as [C-14]lactate from brain labeled with [6-C-14]glucose during times up to 15 min. CMR(Glc) was measured during 5, 7.5, 10 and 15 min in 60 brain areas. At longer times the loss of C-14 was reflected by lower apparent values of brain CMR(Glc). Arteriovenous measurements across brain revealed no significant loss of [C-14]lactate in normal ratsor rats with bicuculline-induced seizures. It was concluded that the primary form in which C-14 was lost was as (CO2)-C-14. As expected, the rate of (CO2)-C-14 loss was greater in structures with high metabolic rates. The data were analyzed to determine the parameters necessary to rectify the data so that uniform values of CMR(Glc) were obtained up to 15 min. Tables were made to predict the degree of C-14 loss as well as the C-14-metabolites/[6-C-14]glucose ratio as a function of timeand metabolic rate. These tables can be used to plan the maximum and minimum experimental times for optimal results.

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Documento generato il 22/01/20 alle ore 18:41:29