Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
FUNCTIONAL EXPRESSION OF A MOUSE GROWTH-HORMONE RECEPTOR CDNA IN TRANSFECTED MOUSE L-CELLS
Autore:
ZHOU YH; XU BX; WANG XZ; CHEN WY; KOPCHICK JJ;
Indirizzi:
OHIO UNIV,DEPT BIOL SCI,MOLEC & CELLULAR BIOL PROGRAM ATHENS OH 45701 OHIO UNIV,DEPT BIOL SCI,MOLEC & CELLULAR BIOL PROGRAM ATHENS OH 45701 OHIO UNIV,EDISON ANIM BIOTECHNOL CTR ATHENS OH 45701
Titolo Testata:
Receptor
fascicolo: 3, volume: 4, anno: 1994,
pagine: 143 - 155
SICI:
1052-8040(1994)4:3<143:FEOAMG>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING-PROTEIN; TYROSINE KINASE; 3T3-F442A FIBROBLASTS; EXTRACELLULAR DOMAIN; TRANSGENIC MICE; GH; PHOSPHORYLATION; STIMULATION; SERUM; IDENTIFICATION;
Keywords:
MOUSE GROWTH HORMONE RECEPTOR CDNA; MOUSE L CELLS; GH ANTAGONIST; PROTEIN TYROSYL PHOSPHORYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
Y.H. Zhou et al., "FUNCTIONAL EXPRESSION OF A MOUSE GROWTH-HORMONE RECEPTOR CDNA IN TRANSFECTED MOUSE L-CELLS", Receptor, 4(3), 1994, pp. 143-155

Abstract

A cDNA encoding a full-length mouse (m) growth hormone receptor (GHR)derived from 3T3 F442A cells was amplified by RT-PCR and cloned into a mammalian expression vector. A mouse L cell:line (mGHR1.6), which expresses high levels of full-length mGHR, was established. A mGHR-specific mRNA of approx 2.8 kb was found in these cells. Ligand binding studies showed that mGHR 1.6 cells were capable of binding I-125-hGH Witha dissociation constant (K-d) of 2.9 +/- 0.13 nM. Scatchard analysis indicated that mGHR1.6 cells had only a single class of mGHR and possessed approx 128,000 GH specific binding sites per cell. Affinity crosslinking studies showed that the recombinant mGHR possessed an apparentmolecular mass of 105 kDa. In addition, mGHR1.6 cells responded to growth hormones (GHs) from several species. Two proteins, pp92 and pp95,were found to be tyrosyl phosphorylated following GH treatment. An hGH antagonist, hGH-G120R, inhibited GH-induced phosphorylation of both pp92 and pp95 in a dose-dependent manner. This cell line may be used as an in vitro model in the studies of GH signal transduction and in the screening of GH analogs for biological activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 15:54:52