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Titolo:
HEXOSE-MONOPHOSPHATE SHUNT ACTIVITY IN INTACT PLASMODIUM-FALCIPARUM-INFECTED ERYTHROCYTES AND IN FREE PARASITES
Autore:
ATAMNA H; PASCARMONA G; GINSBURG H;
Indirizzi:
HEBREW UNIV JERUSALEM,INST LIFE SCI,DEPT BIOL CHEM IL-91904 JERUSALEMISRAEL HEBREW UNIV JERUSALEM,INST LIFE SCI,DEPT BIOL CHEM IL-91904 JERUSALEMISRAEL UNIV TURIN,SCH MED,DIPARTIMENTO GENET BIOL & CHIM MED I-10126 TURIN ITALY
Titolo Testata:
Molecular and biochemical parasitology
fascicolo: 1, volume: 67, anno: 1994,
pagine: 79 - 89
SICI:
0166-6851(1994)67:1<79:HSAIIP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PENTOSE-PHOSPHATE PATHWAY; MALARIA PARASITE; OXIDATIVE STRESS; HOST ERYTHROCYTE; OXIDANT STRESS; GLUCOSE-6-PHOSPHATE-DEHYDROGENASE; BERGHEI; CELL; METABOLISM; CULTURE;
Keywords:
MALARIA; PLASMODIUM FALCIPARUM; OXIDATIVE STRESS; HEXOSE MONOPHOSPHATE SHUNT; NADPH; COMPARTMENT ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
H. Atamna et al., "HEXOSE-MONOPHOSPHATE SHUNT ACTIVITY IN INTACT PLASMODIUM-FALCIPARUM-INFECTED ERYTHROCYTES AND IN FREE PARASITES", Molecular and biochemical parasitology, 67(1), 1994, pp. 79-89

Abstract

The hexose monophosphate shunt (HMS) produces NADPH for reductive antioxidant protection and for metabolic regulation, as well as ribose-5-phosphate needed for the synthesis of nucleic acids. Since malaria-infected red blood cells (RBC) are under endogenous oxidant stress, it was interesting to determine HMS activity in intact infected cells, as well as in free parasites. HMS activity was determined by measuring theevolution of (CO2)-C-14 from D-[1-C-14]glucose in normal RBC, in intact Plasmodium falciparum-infected RBC (IRBC) and in free parasites. The HMS activity of IRBC was found to be 78 times higher than that of normal RBC. This activity increased with parasite maturation from the ring stage toward the trophozoite stage, and declined at the schizont stage. The HMS activity of the parasite contributes 82% of the total observed in the intact IRBC, and that of the host cell is increased some 24-fold. The increased reducing capacity of IRBC and free parasites were also evidenced by the larger ability for the reductive accumulationof methylene blue. Since the endogenous oxidative stress is produced by the parasite digestion of the host cell's hemoglobin inhibition of this process with protease inhibitors, by alkalinization of the parasite's food vacuole, or by the application of antimalarial drugs, resulted in 20-44% inhibition of IRBC HMS activity. A simular inhibition wasobserved in the presence of scavengers of oxidative radicals, uric and benzoic acids. These inhibitors had only a minor effect on the HMS activity of free parasites D-[1-C-14]glucose and D-[6-C-14]glucose contributed equally to newly synthesized nucleic acids, suggesting that ribose-5-phosphate needed for this synthesis is contributed by the non-oxidative activity of HMS. These results imply that a major portion of parasite HMS activity an the activated HMS of the host cell are devoted to counteract the endogenously generated oxidative stress.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 13:50:45