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Titolo:
RETROVIRAL IMMORTALIZATION OF PHAGOCYTIC AND DENDRITIC CELL CLONES ASA TOOL TO INVESTIGATE FUNCTIONAL-HETEROGENEITY
Autore:
LUTZ MB; GRANUCCI F; WINZLER C; MARCONI G; PAGLIA P; FOTI M; ASSMANN CU; CAIRNS L; RESCIGNO M; RICCIARDICASTAGNOLI P;
Indirizzi:
UNIV MILAN,CNR,CTR CYTOPHARMACOL,VIA VANVITELLI 32 I-20129 MILAN ITALY UNIV MILAN,CNR,CTR CYTOPHARMACOL I-20129 MILAN ITALY
Titolo Testata:
Journal of immunological methods
fascicolo: 1-2, volume: 174, anno: 1994,
pagine: 269 - 279
SICI:
0022-1759(1994)174:1-2<269:RIOPAD>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONONUCLEAR PHAGOCYTES; BONE-MARROW; MACROPHAGES; ACTIVATION; ANTIGEN; DIFFERENTIATION; GENERATION; EXPRESSION; GENE;
Keywords:
RETROVIRAL IMMORTALIZATION; MONONUCLEAR PHAGOCYTE; MOUSE MACROPHAGE; MICROGLIA; DENDRITIC CELL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
M.B. Lutz et al., "RETROVIRAL IMMORTALIZATION OF PHAGOCYTIC AND DENDRITIC CELL CLONES ASA TOOL TO INVESTIGATE FUNCTIONAL-HETEROGENEITY", Journal of immunological methods, 174(1-2), 1994, pp. 269-279

Abstract

We have developed a method to generate immortalized phagocytic and dendritic cell clones from various mouse tissues such as spleen, thymus,brain and bone marrow. The clones were phenotypically characterized and shown to retain the ability to respond to immune or inflammatory signals, e.g., IFN-gamma. Functional cytokine activity and nitric oxide production were maintained in activated macrophages, microglial and dendritic cell clones. Immune functions, such as antigen presentation was exhibited by all clones whereas tissue-specific properties such as the ability to respond to corticotropin-releasing hormone and produce beta-endorphin was shown in microglial cell clones but not in macrophage cell clones, indicating that heterogeneity of cells of the mononuclear-phagocytic lineage can be maintained in vitro after the immortalization procedure. Moreover, the continuous proliferation of the clones could be inhibited by various stimuli and further differentiation of the cells could be achieved in vitro.

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Documento generato il 28/11/20 alle ore 21:35:35