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Titolo:
LOCALIZATION OF NUMA PROTEIN ISOFORMS IN THE NUCLEAR MATRIX OF MAMMALIAN-CELLS
Autore:
ZENG CQ; HE DC; BRINKLEY BR;
Indirizzi:
BAYLOR COLL MED,DEPT CELL BIOL,1 BAYLOR PLAZA HOUSTON TX 77030 BAYLOR COLL MED,DEPT CELL BIOL HOUSTON TX 77030
Titolo Testata:
Cell motility and the cytoskeleton
fascicolo: 2, volume: 29, anno: 1994,
pagine: 167 - 176
SICI:
0886-1544(1994)29:2<167:LONPII>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOTIC APPARATUS PROTEIN; MONOCLONAL-ANTIBODY; ELECTRON-MICROSCOPY; CORE FILAMENTS; SPN ANTIGEN; PTK2 CELLS; MITOSIS; SPINDLE; ORGANIZATION; DNA;
Keywords:
NUMA; SPINDLE; NUCLEAR MATRIX; CORE FILAMENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
C.Q. Zeng et al., "LOCALIZATION OF NUMA PROTEIN ISOFORMS IN THE NUCLEAR MATRIX OF MAMMALIAN-CELLS", Cell motility and the cytoskeleton, 29(2), 1994, pp. 167-176

Abstract

Using a monoclonal antibody 2D3 generated against a kinetochore-enriched human chromosome preparation, we identified a high molecular mass protein with nuclear staining in interphase and polar staining of the pericentriolar region in the mitotic spindle. Initially termed centrophilin, this protein associates with the minus-ends of spindle microtubules (MT) and appears to be important in spindle organization [Toussonet al., 1991: J. Cell Biol. 112:427-440]. Comparison of a partial cDNA sequence obtained for centrophilin with the full length cDNA sequence of nuclear mitotic apparatus protein (NuMA) [Compton et al., 1992: J. Cell Biol. 116:1395-1408; Yang et al., 1992: J. Cell Biol. 116:1303-1317] has indicated that NuMA and centrophilin are the same protein. Using a polyclonal NuMA antibody, we have provided further evidence that NuMA exists as isoforms as shown by peptide mapping and immunoblots. Sequential fractionation experiments along with immunofluorescence, immunoblotting, and EM immunogold labeling have demonstrated that NuMA isoforms are novel components of nuclear core filaments. Thus, NuMA, along coiled-coil protein, appears to have dual functions in interphase and mitosis during the cell cycle. In interphase, NuMA likely plays a structural role in the nucleoskeleton that may be important in nuclear organization and functions, whereas in mitosis, NuMA appears to be associated with spindle MT organization and chromosome positioning. (C) 1994 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/10/20 alle ore 01:53:43