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Titolo:
EVALUATION OF DEFINED ANTIGEN VACCINES AGAINST SCHISTOSOMA-BOVIS AND SCHISTOSOMA-JAPONICUM IN BOVINES
Autore:
BASHIR M; BICKLE Q; BUSHARA H; COOK L; SHI FH; HE DA; HUGGINS M; LIN JJ; MALIK K; MOLONEY A; MUKHTAR M; PING Y; XU ST; TAYLOR M; SHI YC;
Indirizzi:
UNIV LONDON LONDON SCH HYG & TROP MED,DEPT MED PARASITOL,KEPPEL ST LONDON WC1E 7HT ENGLAND UNIV LONDON LONDON SCH HYG & TROP MED,DEPT MED PARASITOL LONDON WC1E 7HT ENGLAND UNIV KHARTOUM,FAC VET SCI,DEPT PATHOL KHARTOUM SUDAN CHINESE ACAD AGR SCI,INST ANIM PARASITOL SHANGHAI PEOPLES R CHINA UNIV KHARTOUM,FAC VET SCI,DEPT PREVENT MED KHARTOUM SUDAN
Titolo Testata:
Tropical and geographical medicine
fascicolo: 4, volume: 46, anno: 1994,
pagine: 255 - 258
SICI:
0041-3232(1994)46:4<255:EODAVA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ULTRAVIOLET-ATTENUATED CERCARIAE; INTEGRAL MEMBRANE-PROTEIN; IRRADIATED SCHISTOSOMULA; MOLECULAR-CLONING; PASSIVE TRANSFER; A AUTOANTIGEN; MANSONI; VACCINATION; MICE; IMMUNIZATION;
Keywords:
SCHISTOSOMA BOVIS; SCHISTOSOMA JAPONICUM; VACCINES; BOVINES; SUDAN; CHINA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
M. Bashir et al., "EVALUATION OF DEFINED ANTIGEN VACCINES AGAINST SCHISTOSOMA-BOVIS AND SCHISTOSOMA-JAPONICUM IN BOVINES", Tropical and geographical medicine, 46(4), 1994, pp. 255-258

Abstract

Our objective is to contribute to the development of defined antigen vaccines for schistosomiasis by evaluating the protective efficacy of Schistosoma bovis and S. japonicum antigens in their natural bovine hosts. Antigens under evaluation include some already identified as vaccine candidates: glutathione S transferases (GSTs); KLH, which shares protective epitopes with the protective antigen GP38 of S. mansoni; andSj23, the analogue of the vaccine candidate Sm23 antigen, In another approach, since crude freeze/thaw schistosomular antigen. plus BCG(F/Tvaccine) has proved protective against S. japonicum in bovines, as itwas against S. mansoni in mice, we are carrying out further evaluations both of this crude antigen and of recombinant derived paramyosins, In a third line of work, novel vaccine candidate antigens identified by screening our cDNA libraries with various passively protective animal sera are being evaluated in animal experiments. In the Sudan we haveshown that vaccination of calves with either native S. bovis GSTs or KLH induces high levels of fecundity suppression without causing a significant reduction in adult worm recoveries. Therefore, recombinant-derived S. bovis 28kD GST is now being evaluated, as are the effects of combined GST/KLH vaccination, In China, sheep have been vaccinated with either S. japonicum GSTs, with KLH, or with the F/T vaccine, as a prelude to trials in bovines, As judged by adult worm recoveries, each type of vaccine induced significant protection, and there was also evidence, particularly with the GST and F/T vaccines, of fecundity-suppressive effects. As with the S. bovis/cattle system therefore, both GST and KLH showed protective effects against S. japonicum in sheep. With the latter model, however, both adult worm reductions and fecundity reductions were shown, whereas with S. bovis the vaccines induced strong anti fecundity effects but no worm reductions. Two irradiated vaccine-dominant antigens of S. japonicum have been cloned and sequenced, IVGS3, which shows approximately 80% identity with S. mansoni calreticulinand IVRB4, shown to be homologous to calcium activated neutral protease from S. mansoni. Several antigens have been produced by cloning andexpression of S. japonicum cDNA: a fragment of S. japonicum paramyosin; various regions including the large hydrophilic domain of Sj23; both the 26kD & 28kD S. japonicum GST isoforms. These antigens are currently being tested in protection experiments in mice, Most of these recombinant antigens and also purified native paramyosin are also available for large animal experiments in China.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 23:44:49